Unusual nucleoside triphosphate donors for nucleoside kinases: 3'-deoxyadenosine-2'-triphosphate and 2'-deoxyadenosine-3'-triphosphate

• Autorzy: Krawiec K. , Kierdaszuk B. , Kalinichenko E.N. , Mikhailopulo I.A. , Shugar D.
• Języki publikacji: EN

Abstrakty

EN

Two non-conventional analogues of ATP, 3'-deoxyadenosine-2'-triphosphate (3'-d-2'-ATP) and 2'-deoxyadenosine-3'-triphosphate (2'-d-3'-ATP), the syntheses of which are described, were examined as potential phosphate donors for the nucleoside kinases: 2'-deoxycytidine kinase (dCK), cytosolic thymidine kinase (TK1) and mitochondrial thymidine kinase (TK2). The reactions were monitored by means of a mixture of [γ-32P]ATP and cold analogue, andór with the use of 3H-labelled acceptors and cold donor. With dCK, using equimolar mixtures of ATP with each analogue, and dC as acceptor, phosphate transfer from 3'-d-2'-ATP and 2'-d-3'-ATP amounted to 34% and 14%, respectively. With each analogue used alone (each at concentration of 100 μM), phosphate transfer from 3'-d-2'-ATP was 55% that from ATP, and from 2'-d-3'-ATP 16%. With human TK2, and equimolar mixtures of [γ-32P]ATP with each of the analogues, and 1 μM dT as acceptor, there was no detectable transfer from either analogue. But, when each analogue was used alone, phosphate transfer attained 11% and 5%, respectively, that for ATP alone. With the low affinity form of human TK1, and dT as acceptor, only low phosphate transfer occurred with either analogue used alone. Both compounds exhibited Michaelis-Menten kinetics (with significantly lower Vmax than ATP), while ATP exhibited cooperative kinetics with all three kinases.

Słowa kluczowe

EN

3'-deoxyadenosine-2'-triphosphate; nucleoside kinase; nucleoside triphosphate; 2'-deoxyadenosine-3'-triphosphate; phosphorylation; phosphate donor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.87-94

Twórcy

autor

Krawiec K.

• University of Warsaw, F.Zwirki i S.Wigury 93, 02-089 Warsaw, Poland

autor

Kierdaszuk B.

autor

Kalinichenko E.N.

autor

Mikhailopulo I.A.

autor

Shugar D.

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