Blood leucocyte responses in rats vaccinated with cDNA encoding glutathione-s-transferase of Fasciola hepatica

• Autorzy: Wedrychowicz H , Jedlina-Panasiuk L. , Szymanski P. , Bienkowska-Szewczyk K.
• Języki publikacji: EN

Abstrakty

EN

Changes in blood leucocyte levels were investigated in Spraque-Dowley rats vaccinated with cDNA or protein of glutathione S-transferase (GST) of F. hepatica and subsequently challenged with metacercariae of the liver fluke. The analysis of the leucocyte responses measured in vaccinated rats suggests that the form of antigen used for vaccination intluenced dynamics of white blood cell response to the fluke infection. The most clear differences were observed in neutrophil and eosinophil levels. The weakest reaction of these cells to the challenge infection was observed in rats vaccinated twice with cDNA. In contrast, in rats which received the first antigen dose as cDNA and the second vaccination with GST protein, both neutrophil and eosinophil responses were much higher, especially at 5 and 9 WAI.

PL

Badano reakcję leukocytów krwi szczurów Spraque-Dowley immunizowanych cDNA lub białkiem transferazy S-glutationowej (GST) F. hepatica na inwazję metacerkarii tej przywry. Zwierzęta grupy 3 otrzymały pierwszą dawkę antygenu w formie cDNA a drugą w postaci białka. Próbki krwi do badań pobrano w dniu zarażenia a następne w 1, 5 i 9 tygodniu po zarażeniu (tpz). U szczurów szczepionych cDNA zaobserwowano statystycznie istotne obniżenie liczby limfocytów poczynając od 1 tpz. Największe różnice zaobserwowano w reakcji granulocytów. Szczury immunizowane dwukrotnie cDNA wykazywały najmniejszą eozynofilię zaś u szczurów grupy 3, wystąpiła w 9 tpz eozynofilia i neutrofilia silniej wyrażona niż u szczurów nie immunizowanych.

Słowa kluczowe

EN

glutathione s-transferase; blood; Fasciola hepatica; leucocyte level; vaccinated rat; cDNA; rat; leucocyte

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 4
• Opis fizyczny: p.551-557,fig.,ref.

Twórcy

autor

Wedrychowicz H

• Polish Academy of Sciences, 00-818 Warsaw, Twarda 51-55, Poland

autor

Jedlina-Panasiuk L.

autor

Szymanski P.

autor

Bienkowska-Szewczyk K.

Bibliografia

• BENNETT E.E., HUGHES D. L., HARNESS E. 1980. Fasciola hepatica: Changes in tegument killing of adult flukes surgically transferred to sensitized rats. Parasit. Immunol. 2, 38-55.
• COWDERY J. S., CHACE J. C., YI, A., KRIEG A.M., 1996. Bacterial DNA induces NK cells to produce IFN-y in vivo and increases the toxicity of lipopolysaccharides. J. Immunol. 156: 4570- 4575.
• DOY T. G., HUGHES D. L., HARNESS E. 1978. Resistance of rats to reinfection with Fasciola hepatica and possible involvement of intestinal eosinophil leucocytes. Res. Vet. Science 25: 41-44.
• HALPERN M.D., KURLANDER R. J., PISETSKY D.S. 1996. Bacterial DNA induces murine interferon-γ production by stimulation of interleukin-12 and tumor necrosis factor-α. Cell.Immunol. 167: 72-78.
• KLINMAN D. M., YI A.K., BEAUCAGE S. L., CONOVER, J., KRIEG, A. M. 1996. CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon γ. PNAS 93: 2879-2883.
• KEEGAN P. S., TRUDGETT A. 1992. Fasciola hepatica in the rat: immune responscs associated with the development of resistance to infection. Paras. Immunol. 14: 657-669.
• MILBOURNEE. A., HOWELL M. J. 1990. Eosinophil responses to Fasciola hepatica in rodents. Int. J. Parasitol. 20: 705-708.
• POITOU I., BAEZA E., BOULARD C. 1993. Kinetic responses of parasite-specific antibody isotypes, blood leucocyte patiem and lymphocyte subsets in rats during primary infestation with Fasciola hepatica. Vet. Parasitol. 49: 179-190.
• RAZ E., TIGHE H., SATO Y., CORR M., DUDLER J. A., ROMAN. M., SWAIN S. L., SPIEGELBERG H. L., CARSON D.A., 1996. Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. PNAS 93, 5141-5145.
• SMOOKER P. M., STEEPER K. R., DREW D. R., STRUGNELL R. A., SPITHILL T.W. 2000. Humoral responses in mice following vaccination with DNA encoding glutathione S-transferase of Fasciola hepatica: effects of made of vaccination and the cellular compartment of antigen expression. Parasit. Immunol. 21: 357-364.
• SZYMAŃSKI P., BIEŃKOWSKA-SZEWCZYK K., SZEWCZYK B. 2000. Cloning and overproduction of Fasciola hepatica GST in a Baculovirus expression system bac-to-bac. Mat. 3rd Conference on Molecular Biology in diagnostics of infectious diseases and Biotechnology, Warsaw, 9 Dec. 2000: 109-114.