Plant regeneration from protoplasts obtained from mesophyll leaf tissue of Lycopersicon glandulosum C.H.Mull.

• Autorzy: Lech M. , Pindel A. , Miczynski K.
• Języki publikacji: EN

Abstrakty

EN

High yields of viable protoplasts were obtained from the mesophyll leaf tissue of young seedlings of Lycopersicon glandulosum grown in vitro at 80 µmol s-1m-2 irradiance and 23°-24°C. The isolated protoplasts were cultured first on a modified semiliquid medium of Tan et al. (1987 b), on which they formed microcalli, and afterwards on a greening medium consisting of MS micro/macro nutrients, Nitsch vitamins and 0.5 mg/l BAP + 0.05 mg/l NAA. The green budding calli were in turn transferred to the same medium with BAP and NAA replaced by 2.0 mg/l zeatin + 0.1 mg/l IAA, on which they developed shoots. The regenerated shoots were rooted in 1/3 MS medium without growth regulators, and subsequently planted into pots in the greenhouse. The whole process of regeneration from the moment of protoplast isolation up to the flowering of regenerated plants lasted ~6 months. The regenerated tomatoes were morphologically identical to their parental source plants.

Słowa kluczowe

EN

leaf tissue; plant regeneration; growth regulator; protoplast; Lycopersicon glandulosum; mesophyll cell; regeneration; in vitro; tomato

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1997
• Tom: 39
• Opis fizyczny: p.51-54,fig.

Twórcy

autor

Lech M.

• Agricultural University, Al.29 Listopada 54, 31-425 Krakow, Poland

autor

Pindel A.

autor

Miczynski K.

Bibliografia

• Frearson, E. M., J. B. Power, and E. C. Cocking. 1973. The isolation, culture and regeneration of Petunia leaf protoplasts. Developmental Biology 33: 130-137.
• Koblitz, H. 1991. Protoplast culture and somatic hybridization in Lycopersicon. In: G. Kalloo [ed.], Genetic improvement of tomato, 247-258. Springer Verlag, Berlin.
• Lech, M., K. Miczyński, and A. Pindel. 1996. Comparison of regeneration potential in tissue cultures of primitive and cultivated tomato species (Lycopersicon sp.). Acta Societatis Botanicorum Poloniae 65: 53-56.
• Locy, R. D. 1983. Callus formation and organogenesis by explants of six Lycopersicon species. Canadian Journal of Botany 61: 172-179.
• Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
• Niedz, R. P., S. M. Rutter, L. W. Handley, and K. C. Sink. 1985. Plant regeneration from leaf protoplasts of six tomato cultivars. Plant Science 39: 199-204.
• Nitsch, J. P., and C. Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.
• Patil. R. S., M. R. Davey, and J. B. Power. 1994. Highly efficient plant regeneration from mesophyll protoplasts of indian field cultivars of tomato (Lycopersicon esculentum). Plant Cell, Tissue and Organ Culture 36: 225-258.
• Rudas, V. A., A. V. Zavgorodnyaya, N. N. Cherep, Y. J. Ratushnyak, and Y. Y. Gleba. 1994. Production of initial material for somatic hybridization with cultivated tomato. Abstracts VIIIth International Congress of Plant Tissue and Cell Culture 12-17 June 1994, 101. Firenze.
• Shahin, E. A. 1985. Totipotency of tomato protoplasts. Theoretical and Applied Genetics 69: 235-240.
• Tabaeizadeh, Z., C. Bunisset-Bergounioux, and C. Perennes. 1984. Environmental growth conditions of protoplast course plants: effect on subsequent protoplast division in two tomato species. Physiologiae Vegetale 22: 223-229.
• Tan, M. M. C., H. S. Boerrigter, and A. J. Kool. 1987a. A rapid procedure for plant regeneration from protoplasts isolated from suspension cultures and leaf mesophyll cells of wild Solanum sp. and Lycopersicon pennelli. Plant Science 49: 63-72.
• Tan, M. M. C., E. M. Rietveld, G. A. van Marrevijk, and A. J. Kool. 1987b. Regeneration of leaf mesophyll protoplasts of tomato cultivars (L. esculentum) factors important for efficient protoplast culture and plant regeneration. Plant Cell Reports 6: 172-175.
• Zielińska, F., and G. Bartoszewski. 1994. Ocena reakcji odmian linii pomidora (Lycopersicon esculentum) w kulturze eksplantatów hypokotylowych, liścieniowych i liściowych oraz protoplastów. Potencjalne Zastosowanie Kultur in vitro w Nowoczesnej Hodowli Roślin. Materiały VII Ogólnopolska Konferencja Kultur in vitro, wrzesień 1994, 48. Katowice-Ustrori.