Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay

• Autorzy: Bicka L. , Kuzmak J. , Kozaczynska B. , Plucienniczak A. , Skorupska A.
• Języki publikacji: EN

Abstrakty

EN

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and ex­pressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immuno­blotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for de­tection of BLV antibodies in the infected cattle.

Słowa kluczowe

EN

P24 protein; pol structural gene; cell; gag structural gene; bovine leukemia virus; gag gene cloning; enzootic bovine leukemia; retrovirus; immunoblotting assay; lymphoproliferative disease; cattle; regulatory gene; Escherichia coli; bacteria; env structural gene

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.227-232,fig.

Twórcy

autor

Bicka L.

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kuzmak J.

autor

Kozaczynska B.

autor

Plucienniczak A.

autor

Skorupska A.

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