Changes of nucleotide content in human and rat heart during cardiac surgery and ischemia

• Autorzy: Smolenski R. T. , Skladanowski A.C. , Swierczynski J. , Perko M. , Narkiewicz M. , Zydowo M.M.
• Języki publikacji: EN

Abstrakty

EN

The influence of ischemia on purine nucleotide and their catabolite concentration in human myocardium was investigated during surgery of acquired and congenital heart defects. This was compared with the influence of ischemia on rat heart. Concentrations of adenine and guanine nucleotides and their catabolites were measured in the extracts of heart biopsies taken at the onset of ischemia and at the time of reperfusion. The content of myocardial ATP in human heart decreased from the initial value of 223 ± 1.1 to 14.6 ±1.5 nmol/mg protein and total adenine nucleotide pool decreased from 34.2 ± 1.8 to 27.6 ± 1.5 nmol/mg protein during the operation. Significant increases in myocardial concentrations of purine catabolites were also observed with the most prominent rise in inosine from below 0.5 at the onset of the ischemia to 3.0 ± 0.5 nmol/mg protein at the time of reperfusion. A positive correlation was demonstrated between the concentration of purine catabolites in the heart at the end of ischemia with the decrease of both ATP and the total nucleotide pool. An interesting metabolic specificity of the ischemic human heart appeared to be only a small accumulation of inosine monophospahate (IMP). The increase of IMP in the rat heart after ischemia was several-fold higher.

Słowa kluczowe

EN

purine nucleotide; man; congenital defect; myocardium; ischaemia; acquired defect; heart; nucleotide; rat; cardiac surgery

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1993
• Tom: 40
• Numer: 4
• Opis fizyczny: p.531-538,fig.

Twórcy

autor

Smolenski R. T.

• Academic Medical School of Gdansk, Debinki 1, 80-211 Gdansk, Poland

autor

Skladanowski A.C.

autor

Swierczynski J.

autor

Perko M.

autor

Narkiewicz M.

autor

Zydowo M.M.

Bibliografia

• 1. Achterberg, P.W. (1988) Adenine nucleotides, purine metabolism and myocardial function; in Myocardial Energy Metabolism (de Jong, J.W., ed.) pp. 45 - 52, Nijhoff Publishers, Dordrecht, Boston, Lancaster.
• 2. Olsson, R.A. & Biinger, R. (1987) Metabolic control of coronary blood flow. Progr. Cardio- vasc. Dis. 29,369 - 387.
• 3. Berne, R.M. (1980) The role of adenosine in the control of coronary blood flow. Circ. Res. 47,807 -813.
• 4. Mills, D.C.B., MacFarlane, D.E., Lemmex, B.W.G. & Haslam, R.J. (1983) Receptors for nucleosides and nucleotides on blood platelets; in Regulatory Function of Adenosine (Berne, R.M., Rail, T.W. & Rubio, R., eds.) pp. 277 - 289, Nijhoff Publishers, Hague, Boston, London.
• 5. Gruber, H.G., Hoffer, M.E., McAlister, D.R., Laikind, P.K., Lane, T.A., Schmid-Schoenbein, G.W. & Engler, R.L. (1989) Increased adenosine concentration in blood from ischemic myocar­dium. Circulation 80,1400 - 1411.
• 6. Newby, A.C. (1984) Adenosine and the concept of retaliatory metabolites. Trends Biochem. Sci. 9, 42 - 44.
• 7. Zucchi, R.,Yu,G., Ronca-Testoni,S., Mariani, M. & Ronca, G. (1992) Energy metabolism in myocardial stunning. /. Mol. Cell. Cardiol. 24, 1237-1252.
• 8. Smoleński, R.T., Simmonds, H.A., Garlick, P.B., Veen, G.E. & Chambers, D.J. (1993) Depressed adenosine and total purine catabolite produ­ction in the postischemic rat heart. Cardioscience 4, in press.
• 9. Flameng, W., Van der Vusse, G.J., De Meyere, R., Borgers, M., Sergeant, P., Van der Meersch, E., Ceboers, J. & Suy, R. (1984) Intermittent aortic cross-clamping versus St. Thomas' Hospital cardioplegia in extensive aorta-coronary bypass grafting.J. Thorac. Cardiovasc. Surg. 88,164 -173.
• 10. Teoh, K.H., Mickle, D.A.G., Weisel, R.D., Madonik, M M., Ivanov, J., Harding, R.D., Romaschin, A.D. & Mullen, J.C. (1988) Improving myocardial metabolic and functio­nal recovery after cardioplegic arrest. J. Thorac. Cardiovasc. Surg. 95,788 - 798.
• 11. Solevi, A., Schmidt, W., Jansson, E., Bofim, V. & Kaijser, L. (1987) Adenine nucleotide degra­dation in the human myocardium during cardioplegia. Cardiovasc. Res. 21,358 - 361.
• 12. Flameng, W., DeMeyere, R., Daenen, W., Sergeant, P., Ngalikpima, V., Geboers, J., Suy, R. & Stalpaert, G. (1986) Nifedipine as an adjunct to St.Thomas' Hospital cardioplegia. A double- -blind, placebo-controlled, randomized clinical trial. /. Thorac. Cardiovasc. Surg. 91,723 - 731.
• 13. Van der Vusse, G.J. & van der Veen, F.H. (1988) Myocardial high-energy phosphates during open-heart surgery; in Myocardial Energy Metabolism (de Jong, J.W., ed.) pp. 245 - 255, Nijhoff Publishers, Dodrecht, Boston, Lancaster.
• 14. Hearse, D.J. (1984) Microbiopsy metabolites and paired flow analysis. A new rapid procedure for homogenisation, extraction and analysis of high-energy phosphates and other interme­diates without any errors from tissue loss. Cardiovasc. Res. 18,384 - 390.
• 15. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. /. Biol. Cheitt. 193, 265 -275.
• 16. Smoleński, R.T., Składanowski, A.C., Perko, M. & Żydowo, M.M. (1989) Adenylate degradation product release from the human myocardium. Clin. Chim. Acta 182,63 - 74.
• 17. Kuprianov, V.V., Lakomin, V.L., Kapelko, V.I., Stcinschneider, A.Y., Ruuge, K. & Saks, V.A.(1987) J. Mol. Cell. Cardiol. 19, 729 - 740.
• 18. Humphrey, S.M., Hollis, D.G. & Seelye, R.N. (1985) Myocardial adenine pool depiction and recovery of mechanical function following ischemia. Am. /. Physiol. 248, H644 - H651.
• 19. Meghji, P., Middleton, K.M. & Newby, A.C.(1988) Absolute rates of adenosine formation during ischaemia in rat and pigeon hearts. Biochem. /. 249,695 - 703.
• 20. Van Belle, H., Wynants, J. & Gossens, F. (1985) Formation and release of nucleosides in the ischemic myocardium. Basic Res. Cardiol. 80,653 -660.
• 21. Swain, J.L., Sabina, R.L., McHalle, P.A., Greenfield, J.C. & Holmes, E.W. (1982) Prolonged myocardial nucleotide depiction after brief ischemia in the open chest dog. Am. J. Physiol. 242, H818 - H826.
• 22. Smoleński, R.T., de Jong, J.W., Jannsen, M., Lachno, D R., Żydowo, M M., Tavenier, M., Huizer, T. & Yacoub, M.H. (1993) Formation and breakdown of uridine in ischemic heart. J. Mol. Cell. Cardiol. 25,67 - 74.
• 23. Ledingham, S., Katayama, O., Lachno, D., Patel, N. & Yacoub, M.H. (1990) Beneficial effect of adenosine during reperfusion following pro­longed cardioplegic arrest. Cardiovasc. Res. 24, 247 - 253.24. Zimmer, H.G. & Ibel, H. (1984) Ribose accelcratcs the repletion of the ATP pool during recovery from reversible ischemia in the myocardium. /. Mol. Cell. Cardiol. 16,863 - 866.
• 25. Swain, J., Hines, J.J., Sabina, R.L. & Holmes, E.W. (1982) Accelerated repletion of ATP and GTP pools in postischemic canine myocardium using a precursor of purine de novo synthesis. Circ. Res. 51,102 -105.
• 26. De Jong, J.W., van der Meer, P., van Loon, H., Owen, P. & Opie, L.H. (1990) Adenosine as adjunct to potassium cardioplegia: effect on function, energy metabolism and electro- physiology. J. Thorac. Cardiovasc. Surg. 100,445 - 454.
• 27. Van Belle, H. (1993) Nucleoside transport inhibition: a therapeutic approach to cardio- protection via adenosine ? Cardiovasc. Res. 27,68 -76.
• 28. Składanowski, A.C., Sala, G B. & Newby, A.C. (1989) Inhibition of IMP-specific cytosolic 5'-nucleotidase and adenosine formation in rat polymorphonuclear leucocytes by 5'-deoxy- -5'-isobutylthio derivatives of adenosine and inosine. Biochem. ]. 262,203 - 208.