Ocena przydatnosci wybranych markerow wirulencji do identyfikowania chorobotworczych szczepow paleczek Yersinia enterocolitica. IV. Geny myfA i ureC

• Autorzy: Gierczynski R. , Jagielski M. , Rastawicki W.
• Języki publikacji: PL

Abstrakty

PL

Badano występowanie genów związanych z wytwarzaniem ureazy (ureC) i fimbrii Myf (myfA) u pałeczek Yersinia. Stwierdzono, że gen myfA związany z wytwarzaniem fimbrii Myf może być przydatny w diagnostyce mikrobiologicznej do identyfikowania chorobotwórczych pałeczek Y. enterocolitica, które utraciły plazmid wirulencji pYV. Natomiast gen ureC uznany został za nieprzydatny do tego celu, ze względu na jego występowanie w genomie niechorobotwórczych pałeczek Y. enterocolitica należących do biotypu 1A oraz pałeczek J', intermedia i Y. kristensenii.

EN

We check by polymerase chain reaction (PCR) the presence of gene ureC and myfA, encoding subunits of urease and Myf fimbriae, among clinical and food-originated strains of Yersinia to determine their usefulness as molecular virulence markers of Y. enterocolitica. The examinations were done on 130 clinical strains of Y. enterocolitica O:3/4 isolated in Poland from humans. All strains were obtained from stool and possessed the virulence plasmid pYV. In addition 40 isogenic, plasmid-cured strains were tested. The 52 strains including Y. enterocolitica (biotype 1A, 4, 2 and 1B), Y. pseudotuberculosis, Y. intermedia, Y. frederiksenii, Y. kristensenii, E. coli, Citrobacter, Shigella and Salmonella were used as controls. The PCR assay resulted in detection of genes: ureC and myfA in genomic DNA of all 130 tested clinical strains of Y. enterocolitica pYV+, as well as in plasmid cured strains. Furthermore, ureC was found in all tested strains of Y. enterocolitica biotype A1 and in one strain of Y. intermedia and Y. kristensenii. In contrast to ureC, myfA was detected only in strains of Y. enterocolitica considered as pathogenic.Obtained results show, gene myfA seems to be the reliable virulence marker of Y. enterocolitica, whereas ureC is not recommended for identification of pathogenic strains of this species.

Słowa kluczowe

PL

diagnostyka mikrobiologiczna; gen ureC; ureaza; Yersinia enterocolitica; fimbrie; gen ail; chorobotworczosc; gen yst; geny; gen myfA

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 4
• Opis fizyczny: s.347-355,tab.,fot.,bibliogr.

Twórcy

autor

Gierczynski R.

• Panstwowy Zaklad Higieny, Warszawa

autor

Jagielski M.

autor

Rastawicki W.

Bibliografia

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