maf1 Mutation alters the subcellular localization of the Mod5 protein in yeast

• Autorzy: Murawski M. , Szczesniak B. , Zoladek T. , Hopper A.K. , Martin N.C. , Boguta M.
• Języki publikacji: EN

Abstrakty

Two forms of Mod5p, a tRNA modification enzyme, are found in three intracellular compartments, mitochondria, cytoplasm and nucleus, but arc encoded by a single MOD5 gene. The two forms of the enzyme, Mod5p-I and Mod5p-II differ at the N-termini and are produced by initiation of translation at different start codons. Mod5p-I does contain a mitochondrial targeting signal and is distributed between mitochondria and cytoplasm, whereas Mod5p-II is found in the cytosol and nucleus (Boguta, M., et al. 1994, Mol. Cell. Biol. 14, 229&-2306). In the present work mutants which mislocalize the Mod5p-I enzyme were isolated. The screen was based on a correlation between the amount of cytosolic protein and the efficiency of tRNA mediated suppression. Identification of mutants is possible because a red pigment accumulates in the cells unable to suppress an ade2-l nonsense allele. The mafl mutant, with an altered intracellular localization of the Mod5p-I protein, was isolated. Immunofluorescence data suggest that the mutation causes mislocalization of the Mod5p-I to the nucleus.

Słowa kluczowe

EN

yeast; nuclear localization; protein; protein sorting; mutation; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 4
• Opis fizyczny: p.441-448,fig.

Twórcy

autor

Murawski M.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Szczesniak B.

autor

Zoladek T.

autor

Hopper A.K.

autor

Martin N.C.

autor

Boguta M.

Bibliografia

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