Oligonucleotide synthesis using the 2-[levulinyloxymethyl]-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives

• Autorzy: Kamaike K. , Takahashi H. , Morohoshi K. , Kataoka N. , Kakinuma T. , Ishido Y.
• Języki publikacji: EN

Abstrakty

EN

A comparative study on the utility of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) and 2-(levulinyloxymethyl)benzoyl (LMBz) protecting groups for the 5'-positions of nucleoside 3'-phosphoramidite derivatives in the oligonucleotide synthesis is presented in terms of the syntheses of TpTpT, TpTpTpT, and UpCpApGpUp-UpGpG. In addition we describe the synthesis, using the LMNBz protecting group, of the CpCpA terminus triplet of tRNAs bearing exocyclic amino groups with 15N-labeling, and the trimer Gp[A*]pG containing 2'-O-(β-D-ribofuranosyl)adenosine ([A*]), the latter of which is found at position 64 in the yeast initiator tRNAMet.

Słowa kluczowe

EN

2'-O-[beta-D-ribofuranosyl]adenosine; oligonucleotide synthesis; 2-[levulinyloxymethyl]benzoyl group; 2-[levulinyloxymethyl]-5-nitrobenzoyl group

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 4
• Opis fizyczny: p.949-976,fig.

Twórcy

autor

Kamaike K.

• Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

autor

Takahashi H.

autor

Morohoshi K.

autor

Kataoka N.

autor

Kakinuma T.

autor

Ishido Y.

Bibliografia

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