Zastosowanie real time PCR z uwzglednieniem przydatnosci w diagnostyce wscieklizny

• Autorzy: Orlowska A , Smreczak M. , Trebas P. , Zmudzinski J.F.

Warianty tytułu

EN

Application of real time PCR including rabies diagnostics

• Języki publikacji: PL

Abstrakty

EN

In recent years a considerable improvement has been reported in the field of molecular biology. New molecular methods have replaced classic diagnostic techniques. Apart from pathogen detection, they also allow pathogen quantification in a relatively short time. For instance, real time PCR, thanks to using DNA-binding fluorophores or labelled oligonucleotide probes, allows the detection of virus and monitoring of each cycle of reaction. The use of complementary probes makes this method sensitive and specific. Real time PCR is widely applied, especially in microbiological and virological laboratories, also for rabies diagnostics. Additionally, it is an excellent tool in biomedical and molecular research. For example, it is used for relative and absolute quantification of gene expression and the identification of mutations that might be a key issue in some diseases. Recently, real time PCR seems to become a standard technique in many diagnostic laboratories.

Słowa kluczowe

PL

zastosowanie; metoda Real Time PCR; diagnostyka; wscieklizna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 11
• Opis fizyczny: s.1280-1282,bibliogr.

Twórcy

autor

Orlowska A

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Smreczak M.

autor

Trebas P.

autor

Zmudzinski J.F.

Bibliografia

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