Relationship between the molecular typing of Campylobacter strains and the prevalence of their virulence genes

• Autorzy: Wieczorek K
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate and compare two genotyping methods for the examination of genetic diversity in C. jejuni and C. coli strains isolated from poultry. Moreover, the molecular identification of putative virulence factors was additionally included to establish a correlation between the isolates. Both typing methods, RAPD-PCR and ERIC-PCR, were found to have a high discriminatory power, D=0.95 and 0.94, respectively. The isolates were clustered to 28 groups by RAPD-PCR and to 29 by ERIC- PCR. Sometimes the ERIC or RAPD assays were able to further discriminate the isolates, which had the same virulence profile generated by the second technique. These PCR-based differentiation methods demonstrated the potential for initial rapid epidemiology studies, and when used in conjunction with distribution of virulence markers provide a high resolution strategy for accurately defining subtypes of Campylobacter.

Słowa kluczowe

EN

genotyping method; genetic diversity; Campylobacter jejuni; Campylobacter coli; poultry; molecular identification; putative factor; virulence gene; virulence marker; virulence profile

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Opis fizyczny: p.193-198,fig.,ref.

Twórcy

autor

Wieczorek K

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Akopayanz N., Bukanov N.O., Westblom T.U., Kresovich S., Berg D.E.: DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 1992, 20, 5137-5142.
• 2. Dingle K.E., Colles F.M., Falush D., Maiden M.C.: Sequence typing and comparison of population biology of Campylobacter coli and Campylobacter jejuni. J Clin Microbiol 2005, 43, 340-347.
• 3. Ertas H.B., Cetinkaya B., Muz A., Ongor H.: Genotyping of broiler-originated Campylobacter jejuni and Campylobacter coli isolates using fla typing and random amplified polymorphic DNA methods. Int J Food Microbiol 2004, 94, 203-209.
• 4. Hernandez J., Fayos A., Ferrus M.A., Owen R.J.: Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products. Res Microbiol 1995, 146, 685-696.
• 5. Hilton A.C., Mortiboy D., Banks J.G., Penn C.W.: RAPD analysis of environmental, food and clinical isolates of Campylobacter spp. FEMS Immunol Med Microbiol 1997, 18, 119-124.
• 6. Höök H., Fattah M.A., Ericsson H., Vågsholm I., Danielsson-Tham M.L.: Genotype dynamics of Campylobacter jejuni in a broiler flock. Vet Microbiol 2005, 106, 109-117.
• 7. Hunter P.R., Gaston M.A.: Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 1988, 26, 2465-2466.
• 8. Iriarte P., Owen R.J.: Repetitive and arbitrary primer DNA sequences in PCR-mediated fingerprinting of outbreak and sporadic isolates of Campylobacter jejuni. FEMS Immunol Med Microbiol 1996, 15, 17-22.
• 9. Madden R.H., Moran L., Scates P.: Frequency of occurrence of Campylobacter spp. in red meats and poultry in Northern Ireland and their subsequent subtyping using polymerase chain reaction-restriction fragment length polymorphism and the random amplified polymorphic DNA method. J Appl Microbiol 1998, 84, 703-708.
• 10. Misawa N., Shinohara S., Satoh H., Itoh H., Shinohara K., Shimomura K., Kondo F., Itoh K.: Isolation of Campylobacter species from zoo animals and polymerase chain reaction-based random amplified polymorphism DNA analysis. Vet Microbiol 2000, 71, 59-68.
• 11. Miwa N., Takegahara Y., Terai K., Kato H., Takeuchi T.: Campylobacter jejuni contamination on broiler carcasses of C. jejuni-negative flocks during processing in a Japanese slaughterhouse. Int J Food Microbiol 2003, 84, 105-109.
• 12. On S.L.: In vitro genotypie variation of Campylobacter coli documented by Pulsed-Field Gel Electrophoretic DNA profiling: implications for epidemiological studies. FEMS Microbiol Lett 1998, 165, 341-346.
• 13. Shi Z.Y., Liu P.Y., Lau Y.J., Lin Y.H., Hu B.S., Tsai H.N.: Comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of Campylobacter jejuni. Diagn Microbiol Infect Dis 1996, 26, 103-108.
• 14. Sopwith W., Birtles A., Matthews M., Fox A., Gee S., Michael Painter M., Regan M., Syed Q., Bolton E.: Campylobacter jejuni Multilocus sequence types in humans, Northwest England, 2003-2004. Emerg Infect Dis 2006, 12, 1500-1504.
• 15. Versalovic J., Koeuth T., Lupski J.R.: Distribution of repetive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991, 19, 6823-6831.
• 16. Wang Y., Huang W.M., Taylor D.E.: Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations. Antimicrob Agents Chemother 1993, 37, 457-463.
• 17. Wassenaar T.M., Geilhausen B., Newell D.G.: Evidence of genomic instability in Campylobacter jejuni isolated from poultry. Appl Environ Microbiol 1998, 64, 1816-1821.
• 18. Wieczorek K., Osek J.: Comparison of two molecular methods for genotyping of Campylobacter sp. isolated from poultry. Bull Vet Inst Pulawy 2008, 52, 523-528.
• 19. Wieczorek K., Osek J.: Identification of virulence genes in Campylobacter jejuni and C. coli isolates by PCR. Bull Vet Inst Pulawy 2008, 52, 211-216.
• 20. Wieczorek K., Osek J.: Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Medycyna Wet 2005, 61, 797-799.
• 21. Wilson D.L., Bell J.A., Young V.B., Wilder S.R., Mansfield L.S., Linz J.E.: Variation of the natural transformation frequency of Campylobacter jejuni in liquid shake culture. Microbiology 2003, 149, 3603-3615.
• 22. Zorman T., Heyndrickx M., Uzunovic-Kamberovic S., Smole Mozina S.: Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina. Int J Food Microbiol 2006, 11, 24-33