PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2003 | 54 | 2 |

Tytuł artykułu

Nitric oxide decreases renal medullary Naplus, Kplus-ATPase activity through cyclic GMP-protein kinase G dependent mechanism

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to investigate the effect of nitric oxide on renal Na+,K+-ATPase and ouabain-sensitive H+,K+-ATPase activities. The study was performed in male Wistar rats. The investigated substances were infused under general anaesthesia into abdominal aorta proximally to the renal arteries. The activity of ATPases was assayed in isolated microsomal fraction. NO donor, S-nitroso-N-acetylpenicillamine (SNAP), infused at doses of 10-7 and 10-6 mol/kg/min decreased medullary Na+,K+-ATPase activity by 29.4% and 45.2%, respectively. Another NO donor, spermine NONOate, administered at the same doses reduced Na+,K+-ATPase activity in the renal medulla by 31.7% and 46.5%, respectively. Neither of NO releasers had any effect on Na+,K+-ATPase in the renal cortex and on either cortical or medullary ouabain-sensitive H+,K+-ATPase. Infusion of NO precursor, L-arginine (100 µmol/kg/min), decreased medullary Na+,K+-ATPase activity by 32.2%, whereas inhibitor of nitric oxide synthase, L-NAME (10 nmol/kg/min), increased this activity by 20.7%. The effect of synthetic NO donors was mimicked by 8-bromo-cGMP and blocked by inhibitors of soluble guanylate cyclase, ODQ or methylene blue, as well as by specific inhibitor of protein kinase G, KT5823. In addition, inhibitory effect of either SNAP or 8-bromo-cGMP on medullary Na+,K+-ATPase was abolished by 17-octadecynoic acid (17-ODYA), which inhibits cytochrome P450-dependent metabolism of arachidonic acid. These data suggest that NO decreases Na+,K+-ATPase activity in the renal medulla through the mechanism involving cGMP, protein kinase G, and cytochrome P450-dependent arachidonate metabolites. In contrast, NO has no effect on Na+,K+-ATPase in the renal cortex and on either cortical or medullary ouabain-sensitive H+,K+-ATPase.

Wydawca

-

Rocznik

Tom

54

Numer

2

Opis fizyczny

p.191-210,fig.

Twórcy

autor
  • Medical University, Jaczewskiego 8, 20-090 Lublin, Poland
autor
autor
autor

Bibliografia

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-ad30cfc4-a7f2-4b16-996d-162b67cae75e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.