Actinomycin D specifically inhibits the interaction between transcription factor Sp1 and its binding site

• Autorzy: Czyz M. , Gniazdowski M.
• Języki publikacji: EN

Abstrakty

EN

The mode of action of many anticancer drugs involves DNA interactions. We here examine the ability of actinomycin D to alter the specific binding of transcription factors Sp1 and NFκB to their DNA sequences. Employing an electrophoretic mobility shift assay, it is shown that actinomycin D inhibits complex formation between nuclear proteins present in the extracts from stimulated human umbilical vein endothelial cells and the Sp1-binding site. Actinomycin D is also able to induce disruption of preformed DNA-protein complexes, pointing to the importance of an equilibrium of three components: actinomycin D, protein and DNA for drug action. The effect of actinomycin D is sequence-specific, since no inhibition is observed for interaction of nuclear proteins with the NFκB binding site. The results support the view that DNA-binding drugs displaying high sequence-selectivity can exhibit distinct effects on the interaction between DNA and different DNA-binding proteins.

Słowa kluczowe

EN

stimulatory protein 1; DNA-protein interaction; anticancer drug; transcription factor; lipopolysaccharide; actinomycin D; drug; DNA binding; endothelium

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.67-73,fig.

Twórcy

autor

Czyz M.

• Medical University of Lodz, W.Lindleya 6, 90-131 Lodz, Poland

autor

Gniazdowski M.

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