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2009 | 65 | 05 |

Tytuł artykułu

Techniki uzyteczne w ocenie swoistej odpornosci komorkowej

Warianty tytułu

EN
Methods useful in the evaluation of specific cell-mediated immunity

Języki publikacji

PL

Abstrakty

EN
In the era of the excessive use of chemotherapeutics, especially antibiotics and an increase of bacterial strains resistance, studies concerning the stimulation of the immune system and evaluation of its activity seem to be particularly important. This article presents a review of the methods useful in the evaluation of cellular immunity. The evaluation of specific cell-mediated immunity may be performed in vivo or in vitro. Delayed-type hypersensitivity assay is one that is possible to make in vivo, while the lymphocyte transformation test, cytometric proliferation assay, evaluation of activation marker expression and analysis of antigen-specific production of cytokines by sensitive lymphocytes T are methods performed in vitro. The evaluation of cell-mediated immunity in immunological studies, especially concerning vaccinology, will be valuable or even essential elements of an experiment.

Wydawca

-

Rocznik

Tom

65

Numer

05

Opis fizyczny

s.306-309,bibliogr.

Twórcy

  • Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

Bibliografia

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  • 2. Caruso A., Licenziati S., Corulli M., Canaris A. D., Francesco De M. A., Fiorentini S., Peroni L., Fallacara F., Dima F., Balsari A., Turano A.: Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation. Cytometry 1997, 27, 71-76.
  • 3. Dorn A. T., Waters W. R., Byers V. M., Pesch B. A., Wannemuehler M. J.: Characterization of mitogen-stimulated porcine lymphocytes using a stable fluorescent dye (PHK2) and multicolor flow cytometry. Vet. Immunol. Immunopathol. 2002, 87, 1-10.
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  • 5. Fachinger V., Schlapp T., Strube W., Schmeer N., Saalmüller A.: Poxvirus-induced immunostimulating effects on porcine leukocytes. J. Virol. 2000, 74, 7943-7951.
  • 6. Givan A. L., Fisher J. L., Waugh M., Ernstoff M. S., Wallace P. K.: A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J. Immunol. Methods 1999, 230, 99-112.
  • 7. Iżycki D.: Zastosowanie cytometrii przepływowej w klinicznej diagnostyce laboratoryjnej. Voice 2006, 2, 8-17.
  • 8. Kristensen C. S., Andreasen M., Ersbøll A. K., Nielsen J. P.: Antibody response in sows and piglets following vaccination against Mycoplasma hyopneumoniae toxigenic Pasteurella multocida and Actinobacillus pleuropneumoniae. Can. J. Vet. Res. 2004, 68, 66-70.
  • 9. Mojžišova J., Hromada R., Paulik S., Ondrašovič M., Bajova V.: Immune response and immunomodulatory effect of levamisole in immunosuppressed dogs vaccinated against parvovirosis. Bull. Vet. Inst. Pulawy 2004, 48, 93-97.
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  • 11. Motubu M., El-Abasy M., Na K., Hirota Y.: Detection of mitogen-induced lymphocyte by bromodeoxyuridine (BrdU) incorporation in chicken. J. Vet. Med. Sci. 2002, 64, 377-379.
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  • 13. Nowell P. C.: Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960, 20, 462-466.
  • 14. Pejsak Z., Markowska-Daniel I.: Randomised, placebo-controlled trial of a live vaccine against porcine reproductive and respiratory syndrome in sows on infected farms. Vet. Rec. 2006, 158, 475-478.
  • 15. Raymond C. R., Wilkie B. N.: Th-1/Th-2 type cytokine profiles of pig T-cells cultured with antigen-treated monocyte-derived dendritic cells. Vaccine 2004, 22, 1016-1023.
  • 16. Riber U., Jungersen G.: Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O:9 in pigs. Vet. Immunol. Immunopathol. 2007, 116, 13-25.
  • 17. Rustemeyer T., De Ligter S., Von Blomberg B. M. E., Frosch P. J., Scheper R. J.: Human T lymphocyte priming in vitro by haptenated autologous dendritic cells. Clin. Exp. Immunol. 1999, 117, 209-216.
  • 18. Saker K. E., Kalnitsky J., Gogal R. M., Ward D. L.: Evaluation of a nonradioactive colorimetric assay for analysis of lymphocyte proliferation in healthy cats. Am. J. Vet. Res. 2001, 62, 567-571.
  • 19. Sanchez A., Maruta C., Sato M., Ribeiro R., Zomignan C., Nunes R., Reis V.: Study on lymphocyte proliferation in nickel sensitive patients. An. Bras. Dermatol. 2005, 80, 149-158.
  • 20. Sathiyaseelan T., Baldwin C. L.: Evaluation of cell replication by bovine T cells in polyclonally activated cultures using carboxyfluorescein succinimidyl ester (CFSE) loading and flow cytometric analysis. Res. Vet. Sci. 2000, 69, 275-281.
  • 21. Stec J., Rachubik J., Szczotka M., Kuźmak J.: Effects of penicillium mycotoxins: citrinin, ochratoxin a, and patulin on in vitro proliferation of bovine lymphocytes. Bull. Vet. Inst. Pulawy 2008, 52, 163-167.
  • 22. Waters W. R., Peschb B. A., Hontecillasa R., Saccoc R. E., Zuckermannd F. A., Wannemuehler M. J.: Cellular immune responses of pigs induced by vaccination with either a whole cell sonicate or pepsin-digested Brachyspira (Serpulina) hyodysenteriae bacterin. Vaccine 2000, 18, 711-719.
  • 23. Waters W. R., Palmer M. V., Pesch B. A. Olsen S. C., Wannemuehler M. J., Whipple D. L.: Lymphocyte subset proliferation responses of Mycobacterium bovis-infected cattle to purified protein derivative. Vet. Immunol. Immunopathol. 2000, 77, 257-273.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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