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Tytuł artykułu

Influence of genotype and medium on culture of immature zygotic embryos of Cucumis sativus L. and Cucumis melo L.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Four genotypes of Cucumis sativus and Cucumis melo were used to make embryo culture. Embryos were excised from unripe fruits 1, 2 and 3 weeks after self-pollination. MS (Murashige and Skoog, 1962) basal medium was supplemented with ascorbic acid, growth regulators and 2% or 3% sucrose. Higher regeneration was achieved on medium with lower concentration of sucrose and phytohormones. Plants were regenerated from immature embryos isolated 1 week after self-pollination for C. sativus (Sm-6514) and 2 weeks after self-pollination for C. melo.

Wydawca

-

Rocznik

Tom

42

Numer

2

Opis fizyczny

p.79-81

Twórcy

autor
  • Palacky University, Slechtitelu 11, 783-71 Olomouc-Holice, Czech Republic

Bibliografia

  • Bogunia H, and Przywara L. 1999. The influence of different carbohydrates on the development of immature zygotic rape embryos (Brassica napus L. cv. Topas). Acta Biologica Cracoviensia Series Botanica 41, suppl. 1: 33.
  • Chen JF, Staub JE, Tashiro Y, Isshiki S, and Miyazaki S. 1997. Successful interspecific hybridization between Cucumis sativus L. and Cucumis hystrix Chakr. Euphytica 96: 413-419.
  • Custers JBM. 1981. Heart shape stage embryos of Cucumis species are more successful in embryo culture than advanced stage embryos. Cucurbit Genetics Cooperative Reports 4: 48-49.
  • Haldaś W, and Przywara L. 1999. In vitro culture of immature embryos of white clover (Trifolium repens L.). Acta Biologica Cracoviensia Series Botanica 41, suppl. 1: 35.
  • Lebeda A, Křistková E, and Kubaláková M. 1996. Interspecific hybridization of Cucumis sativus x Cucumis melo as potential way to transfer resistance to Pseudoperonospora cubensis. In: Gómez-Guillamón ML, Soria C, Cuartero J, Torés JA, and Femández-Munoz R [eds.], Proceedings of the Vlth Eucarpia Meeting on Cucurbit Genetics and Breeding, ‘Cucurbits Towards 2000’, 28-30 May 1996, 31-37. Malaga, Spain.
  • Monnier M. 1995. Culture of zygotic embryos. In: Thorpe TA [ed.], In vitro embryogenesis in plants, 117-153. Kluwer Academic Publishers, Dordrecht.
  • Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 474-497.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-ac5a7882-c4c1-4774-8c52-776e3824e7f6
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