DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response

• Autorzy: Cao W , Hunter R , Strnatka D , McQueen C A , Erickson R P
• Języki publikacji: EN

Abstrakty

EN

Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with these constructs, perhaps due in part to an interferon response as reflected in increases in oligoadenylate synthetase I mRNA levels. Seven out of 8 founders with the U6 promoter generated offspring but only 2 gave positive offspring. Out of 15 founders for H1 promoted constructs, only 4 had positive offspring. When transgenic lines were successfully established, the expression of the targeted genes was variable between animals and was not generally inhibitory.

Słowa kluczowe

EN

interfering RNA; short hairpin RNA; mice; transgenics; NAT1 gene; NAT2 gene; transgenic mice; embryo death; mouse; interferon response; silencing RNA; transgenic mouse; arylamine N-acetyltransferase

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 2
• Opis fizyczny: p.217-225,fig.,ref.

Twórcy

autor

Cao W

• Department of Pediatrics, Angel Charity for Children - Wings for Genetic Research, Steele Memorial Childrens Research Center, 4341B. 1501 N. Campbell Avenue, P.O.Box 245073, Tucson, Arizona 85724-5073, USA

autor

Hunter R

• Department of Pediatrics, Angel Charity for Children - Wings for Genetic Research, Steele Memorial Childrens Research Center, 4341B. 1501 N. Campbell Avenue, P.O.Box 245073, Tucson, Arizona 85724-5073, USA

autor

Strnatka D

• Department of Pediatrics, Angel Charity for Children - Wings for Genetic Research, Steele Memorial Childrens Research Center, 4341B. 1501 N. Campbell Avenue, P.O.Box 245073, Tucson, Arizona 85724-5073, USA

autor

McQueen C A

• Department of Pharmacology and Toxicology

autor

Erickson R P

• Department of Pediatrics, Angel Charity for Children - Wings for Genetic Research, Steele Memorial Childrens Research Center, 4341B. 1501 N. Campbell Avenue, P.O.Box 245073, Tucson, Arizona 85724-5073, USA
• Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona

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