EN
Urea and uraease (U-ase) activity were determined in water samples taken from the surface layers of 17 lakes of different trophic status. Urea concentrations were inversely correlated with the trophic status of the studied lakes and varied from below the detection limit to 25 μmol 1⁻¹. Maximal potential ureolytic activity (Vmax) ranged from 0.2 to 7.0 μmol 1⁻¹ h⁻¹. The highest urea concentrations and the lowest U-ase activities were recorded in the spring, whereas the lowest urea concentrations and the highest rates of urea hydrolysis were observed late in summer, during heavy phytoplankton blooms. Since in the majority of the Great Mazurian Lakes microplankton growth was limited by nitrogen supply, urea was an important N source for both auto- and heterotrophic planktonic microorganisms throughout the growth period. U-ase activity was mainly related to the seston. Only up to 25% of total activity could be attributed to free enzymes dissolved in lake water. In epilimnetic water samples the bulk of the ureolytic activity originated from seston-attached bacteria. However, a positive, statistically significant correlation between ureolytic activity and chlorophyll a (Chla) concentrations suggests that phytoplankton may also be responsible for at least a some of the observed ureolytic activity in the highly eutrophic Great Mazurian Lakes.