Gene expression profiling of lipoarabinomannan-treated mouse macrophage cultures infected with Mycobacterium bovis BCG

• Autorzy: Krzyzowska M , Malewski T. , Hamasur B. , Augustynowicz-Kopec E. , Schollenberger A. , Pawlowski A. , Niemialtowski M.
• Języki publikacji: EN

Abstrakty

EN

The mannosylated lipoarabinomanan (ManLAM) from mycobacterial species possesses strong anti-apoptotic action. Here we examined the ability of ManLAM isolated from Mycobacterium tuberculosis H37Rv to alter expression profiles of apoptosis-related genes in mouse macrophages infected with Mycobacterium bovis BCG Danish strain. ManLAM suppressed BCG-induced apoptosis and activities of caspase -1, -3, -8 and 9. Mouse Apoptosis Gene Array showed that ManLAM significantly down-regulated pro-apoptotic and proinflammatory genes: caspase -1, -3, -7, -8 and -9, TNF-α/TNFSF2, Fas/TNFRSF6, Bax-α, as well as IL-12 p35 and iNOS simultaneously up-regulating anti-apoptotic genes such as Bcl-2 and Mcl-1. The effect of ManLAM was contrary to BCG-induced up-regulation of pro-apoptotic and pro-inflammatory genes and consistent with the functional data.

Słowa kluczowe

EN

lipoarabinomannan; gene expression; apoptosis; Mycobacterium bovis; DNA microarray; human macrophage; apoptosis-related gene; mouse macrophage

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 2
• Opis fizyczny: p.125-133,fig.,ref.

Twórcy

autor

Krzyzowska M

• Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Malewski T.

autor

Hamasur B.

autor

Augustynowicz-Kopec E.

autor

Schollenberger A.

autor

Pawlowski A.

autor

Niemialtowski M.

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