Cytological and genetic evaluation of anther culture-derived doubled haploids in barley

• Autorzy: Szarejko I , Falk D E , Janusz A , Nabialkowska D
• Języki publikacji: EN

Abstrakty

EN

Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.

Słowa kluczowe

EN

cytological analysis; doubled haploid; morphological variation; genotype; barley; genetic evaluation; gametoclonal variation; chlorophyll mutation test; Hordeum vulgare; anther culture

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 4
• Opis fizyczny: p.437-452

Twórcy

autor

Szarejko I

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

autor

Falk D E

• Crop Science Department, University of Guelph, Guelph, Ont., Canada

autor

Janusz A

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

autor

Nabialkowska D

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

Bibliografia

• Baenziger P.S., Peterson C.J., Morris M.M., Mattern P.J. (1989a). Quantifying gametoclonal variation in wheat doubled haploids. In: Current Options for Cereal Improvement: Doubled Haploids. Mutants and Heterosis (M. Maluszynski, ed.). Kluwer Academic Publisher, Dordrecht: 1-9.
• Baenziger P.S., Wesenberg D.M., Smail V.M., Alexander W.L., Schaeffer G.W. (1989b). Agronomic performance of wheat doubled-haploid lines derived from cultivars by anther culture. Plant Breeding 103: 101-109.
• Borrino E.M., Powell W. (1988). Stomatal guard cell length as an indicator of ploidy in microspore-derived plants of barley. Genome 30: 158-160.
• Chen C.C., Kasha K.J., Marsolais A. (1984). Segmentation patterns and mechanisms of genome multiplication in cullured microspores of barley. Can. J. Genet. Cytol. 26: 475-483.
• Datta S.K., Potrykus I. (1989). Artificial seeds in barley: encapsulation of microspore-derived embryos. Theor. Appl. Genet. 77: 820-824.
• Day A., Ellis T.H.N. (1985). Deleted forms of plastic DNA in albino plants from cereal anther culture. Curr. Genet. 9: 671-678.
• Devaux P. (1988). Comparison of anther culture and the Hordeum bulbosum method for the production of doubled haploids in winter barley. II. Variation of chromosome number and seed set in the progeny. Plant Breeding 100: 181-187.
• Finnie S.J., Powell W., Dyer A.F. (1989). The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.). Plant Breeding 103: 110-118.
• Foroughi-Wehr B., Friedt W. (1984). Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor. Appl. Genet. 67: 377-382.
• Friedt W., Breun J., Zuchner S., Foroughi-Wehr B. (1986). Comparative value of androgenetic doubled haploid and conventionally selected spring barley lines. Plant Breeding 97: 56-63.
• Friedt W., Foroughi-Wehr B. (1980). Microspore derived chromosome number and structural variants of barley (Hordeum vulgare L.). Barley. Genet. Newsl. 10: 16-20.
• Friedt W., Foroughi-Wehr B. (1983). Field performance of androgenctic doubled haploid spring barley from F₁ hybrids of Hordeum vulgare. Z. Pflanzenzüchtg. 90: 177-184.
• Fukui K. (1983). Sequential occurrence of mutations in a growing rice callus. Theor. Appl. Genet. 65: 225-230.
• Hunter C.P. (1987). Plant generation method. European Patent Appl. No. 0 245 898 A2: 1-8.
• Idzikowska K., Ponitka A., Zenkteler M., Młodzianowski F. (1981). The first stages of microspore division in anthers of Hordeum vulgare cultured in vitro. Flora 171: 11-22.
• Kasha K.J., Ziauddin A., Cho U.H. (1990). Haploids in cereal improvement: anther and microspore culture. In: Gene Manipulation in Plant Improvement II. Proc. 19th Stadler Genetics Symp. (J.P. Gustafson, ed.). Plenum Press, New York: 213-235.
• Kasha K.J., Ziauddin A., Simion E., Marsolais A.A., Chen Y.R. (1991). Barley and wheat microspore culture. In: Proc. FAO/IAEA Third RCM on "Use of Induced Mutation in Connection with Haploids and Heterosis in Cereals", Pullman, USA, IAEA 312.D2.RC.351.3, Vienna: 11-16.
• Kilian A., Gale M.D. (1991). Induction of RFLPs by mutagenesis. Cereal Res. Commun. 19: 119- 129.
• Kuhlmann U., Foroughi-Wehr B. (1989). Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78-81.
• Lee F.M., Chen C.C. (1987). Nuclear fusion in cultured microsporcs of barley. Plant Cell Rep. 6: 191-193.
• Liang G.H., Xu A., Hoang-Tang (1987). Direct generation of wheat haploids via anther culture. Crop Sci. 27: 336-339.
• Lyne R.L., Bennett R.I., Hunter C.P. (1986). Embryoid and plant production from cultured barley anthers. In: Plant Tissue Culture and Its Agricultural Applications (P.G. Anderson, L.A. Withers, eds.). Butterworths, Guildford: 405-411.
• Maluszynska J., Maluszynski M. (1983). MNUA and MH mutagenic effect after double treatment of barley seeds in different germination periods. Acta Biologica 22: 238-248.
• Marburger J.E., Jauhar P.P. (1989). Agronomic, isozyme, and cytogenetic characteristics of ’Chris’ wheat doubled haploids. Plant Breeding 103: 73-80.
• Marsolais A.A., Kasha K.J. (1985). Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium. Can. J. Bot. 63: 2209-2212.
• Metz S.G., Sharma H.C., Armstrong T.A., Mascia P.N. (1988). Chromosome doubling and aneuploidy in anther-derived plants from two winter wheat lines. Genome 30: 177-181.
• Mix G., Wilson H.M., Foroughi-Wehr B. (1978). The cytological status of plants of Hordeum vulgare L. regenerated from microspore callus. Z. Pflanzenzüchtg. 80: 89-99.
• Nielsen G., Johansen H.B. (1986). Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isozyme loci of 47 reference varieties. Euphytica 35: 717-728.
• Olsen F.L. (1987). Induction of microsporc organogenesis in cultured anthers of Hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res. Commun. 52: 393-404.
• Park S.J., Walsh E.J., Reinbergs E., Song L.S.P., Kasha K.J. (1976). Field performance of doubled haploid barley lines in comparison with lines developed by the pedigree and single seed descent methods. Can. J. Plant Sci. 56: 467-474.
• Powell W., Borrino E.M., Goodall V. (1988). The effect of anther orientation on microspore-derived plant production in barley (Hordeum vulgare L.). Euphytica 38: 159-163.
• Powell W., Caligari P.D.S., Dunwell J.M. (1986). Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Appl. Genet. 72: 458-465.
• Powell W., Hayter A.M., Wood W., Dunwell J.M., Huang B. (1984). Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare cv. Sabarlis. Heredity 52: 19-23.
• Reinbergs E., Song L.S.P., Choo T.M., Kasha K.J. (1978). Yield stability of doubled haploid lines of barley. Can. J. Plant Sci. 58: 929-933.
• Rode A., Hartman C., Benslimane A., Picard E., Quetier F. (1987). Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L.) cv. ’Cesar’. Theor. Appl. Genet. 74: 31-37.
• Rossnagel B.G., Sariah M.A., Kao K.N. (1987). Field evaluation of anther culture derived breeding materials compared to materials developed by the pedigree and single seed descent methods in barley (Hordeum vulgare L.). In: Barley Genetics V, Proc. Fifth Inter. Barley Genet. Symp., Okayama: 997-1004.
• Snape J.W., Simpson E., Parker B.B., Friedt W., Foroughi-Wehr B. (1986). Criteria for the selection and use of doubled haploid systems in cereal breeding programmes. In: Genetic Manipulation in Plant Breeding (W. Horn, V. Jensen, W. Odenbach, O. Schieder, eds.). Walter de Gruyter, Berlin: 217-229.
• Snape J.W., Sitch L.A., Simpson E., Parker B.B. (1988). Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system. Theor. Appl. Genet. 75: 509-513.
• Sorvari S., Schieder O. (1987). Influence of sucrose and melibiose on barley anther cultures in starch media. Plant Breeding 99: 164-171.
• Szarejko I., Kasha K.J. (1991). Induction of anther culture derived doubled haploids in barley. Cereal Res. Commun. 19: 219-237.
• Szarejko I., Polok K., Maluszynski M., Falk D.E. (1991). Isozyme analysis of anther culture derived plants in barley. Cereal Res. Commun. 19: 239-244.
• Thompson D.M., Chalmers K., Waugh R., Forster B.P., Thomas W.T.B., Caligari P.D.S., Powell W. (1991). The inheritance of genetic markers in microspore-derived plants of barley Hordeum vulgare L. Theor. Appl. Genet. 81: 487-492.
• Ullrich S.E., Edmiston J.M., Kleinhofs A., Kudrna D.A., Maatougui M.E.H. (1991). Evaluation of somaclonal variation in barley. Cereal Res. Commun. 19: 245-260.
• Wilson H.M., Mix G., Foroughi-Wehr B. (1978). Early microspore divisions and subsequent formation of microspore calluses at high frequency in anthers of Hordeum vulgare L. J. Exp. Bot. 29: 227-238.
• Yang H.Y., Zhou C. (1982). In vitro induction of haploid plants from unpollinated ovaries and ovules gynogenesis. Theor. Appl. Genet. 63: 97-104.
• Ziauddin A., Simon E., Kasha K.J. (1990). Improved plant regeneration from shed microsporc culture in barley (Hordeum vulgare L.) cv. Igri. Plant Cell Rep. 9: 57-65.