Aktywnosc katalazy po zastosowaniu wybranych inhibitorow ADH i MEOS, badania in vitro w homogenatach watroby szczurow

• Autorzy: Dawidek-Pietryka K. , Dudka J. , Jagiello-Wojtowicz E.
• Języki publikacji: PL

Abstrakty

PL

Prezentowana praca jest kontynuacją badań nad wpływem wybranych inhibitorów ADH i MEOS na pierwszy etap biotransformacji metanolu. Stosowano: 3-amino-1,2,4-triazol, cymetydynę, EDTA, 1,10-fenantrolinę i 4-metylopirazol w reakcji utleniania metanolu w stężeniach 0,05 i O, 10 mol/L Oceniano ich wpływ na aktywność katalazy w homogenatach wątroby szczurów.

EN

In the first pass methanol biotransformation three enzymatic systems: alcohol dehydrogenase (ADH), microsomal alcohol oxidising system (MEOS) linked with cytochrome P-450 and catalase are involved. Because of the toxicity of methanol, which is directly caused by its toxic metabolites, the major task in clinical toxicology is to inhibit each of these enzymes to protect human life. The aim of this investigation was to check the influence of some effective inhibitors of ADH and MEOS: 4-methylpyrazole, Cimetidine, EDTA and 1,10-phenantroline on the activity of catalase with methanol as a substrate and the comparison with 3-amino-1,2,4-triasole Catalase activity in rat hepatic homogenates was measured spectrophotometrically in vitro at physiological pH 7.4 and temp. 37 °С, assaying the degree of methanol oxidation according to Handler and Thurman. The quantity of arising formaldehyde was measured according with the method of Nash. Our results have shown that catalase activity was inhibited to different extents by all investigated compounds at concentrations of 10-3 mol/1, 2 x 10-4 mol/1, 10-4 mol/1, 2 x 10-5 mol/l, 10-5 mol/1. 1,10-Phenantroline was found to be a highly effective inhibitor in comparison with aminotriasole. 4-Methylpyrazole, EDTA, 1,10-phenantroline and aminotriasole are catalase competitive inhibitors and Cimetidine is non-competitive inhibitor. 4-Methylpyrazole has shown higher affinity to the enzyme than aminotriasole.

Słowa kluczowe

PL

szczury; utlenianie; metanol; inhibitory ADH; aktywnosc enzymatyczna; homogenaty; katalaza; inhibitory MEOS; watroba

EN

rat; oxidation; methanol; alcohol dehydrogenase oxidizing inhibitor; enzyme activity; homogenate; catalase; microsomal alcohol oxidizing system; inhibitor; liver

• Wydawca: -
• Czasopismo: Roczniki Państwowego Zakładu Higieny
• Rocznik: 2003
• Tom: 54
• Numer: 4
• Opis fizyczny: s.373-381,tab.,rys.,wykr.,bibliogr.

Twórcy

autor

Dawidek-Pietryka K.

• Akademia Medyczna, Lublin

autor

Dudka J.

autor

Jagiello-Wojtowicz E.