A new diagnostic SSR marker for selection of the Rym4-Rym5 locus in barley breeding

• Autorzy: Tyrka M , Perovic D. , Wardynska A , Ordon F
• Języki publikacji: EN

Abstrakty

EN

Genomic sequence AY661558, representing a part of the ВАС contig of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV/BaYMV), was exploited in order to develop SSR markers for practical barley breeding. Out of 57 SSR motifs found within this sequence, primers were designed and tested for the 5 SSRs with the highest repeat length. The polymorphic SSR marker QLB1 со-segregated with rym4 and rym5 phenotypes in respective high-resolution mapping populations developed for the construction of the original ВАС contig. The primers targeted 2 sites located 756 bp and 5173 bp downstream of the translation initiation factor 4E (Hv-eIF4E). Physical linkage of the QLB1 marker to the Rym4/Rym5 locus was confirmed experimentally on Morex ВАС 519J14, a seed ВAC of Hv-eIF4E, and ВAC 801A11, which is located proximally to Hv-eIF4E. QLB1 revealed 7 alleles in a set of 100 winter barley lines and cultivars. Five alleles were found within 673 advanced breeding lines derived from applied Polish winter barley breeding programmes, which corresponds to a PIC value of 0.684. No recombinants between Rym4/5 and QLB1 were detected, suggesting that QLB1 can be used efficiently in marker-assisted selection of the Hv-eIF4E-mediated bymovirus resistance.

Słowa kluczowe

EN

plant breeding; barley; winter barley; simple sequence repeat; DNA marker; selection; plant pathogen; barley yellow mosaic virus; barley mild mosaic wirus

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 2
• Opis fizyczny: p.127-134,fig.,ref.

Twórcy

autor

Tyrka M

• Laboratory of Population Genetics, Polonia University, Czestochowa, Poland

autor

Perovic D.

• Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources, Erwin-Baur, Quedlinburg, Germany

autor

Wardynska A

• Laboratory of Population Genetics, Polonia University, Czestochowa, Poland

autor

Ordon F

• Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources, Erwin-Baur, Quedlinburg, Germany

Bibliografia

• Chalhoub BA, Thibault S, Laucou V, Rameau C, Höfte H, Cousin R, 1997. Silver staining and recovery of AFLP™ amplification products on large denaturing polyacrylamide gels. BioTechniques 22: 216-220.
• Graner A, Bauer E, 1993. RFLP mapping of the ym4 virus resistance gene in barley. Theor Appl Genet 86: 689-693.
• Graner A, Streng S, Kellermann A, Proeseler G, Schiemann A, Peterka H, Ordon F, 1999a. Molecular mapping of genes conferring resistance to soil-borne viruses in barley: an approach to promote understanding of host-pathogen interactions. J Plant Dis Prot 106: 405-410.
• Graner A, Streng S, Kellermann A, Schemann A, Bauer E, Waugh R, et al. 1999b. Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex. Theor Appl Genet 98: 285-290.
• Harding RM, Boyce AJ, Clegg JB, 1992. The evolution of tandemly repetitive DNA: recombination rules. Genetics 132: 847-859.
• Kanyuka K, McGrarm G, Alhudaib K, Hariri D, Adams MJ, 2004. Biological and sequence analysis of a novel European isolate of Barley mild mosaic virus that overcomes the barley rym5 resistance gene. Arch Virol 149: 1469-1480.
• Konishi T, Ordon F, Furusho M, 2002. Reactions of barley accessions carrying different rym genes to BaYMV and BaMMV in Japan and Germany. Barley Genet Newsl 32: 46-48.
• Le Gouis J, Ordon F, Friedt W, 1994. Isozyme-electrophoresis for chromosomal localization of barley mild mosaic virus resistance genes different from ym4. Barley Genet Newsl 23: 36-39.
• Li Y-C, Korol AB, Fahima T, Beiles A, Nevo E, 2002. Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol Ecol 11: 2453-2465.
• McGrann GRD, Adams MJ, 2004. Investigating resistance to Barley mild mosaic virus. Plant Pathology 53: 161-169.
• Milligan BG, 1992. Plant DNA isolation. In: Hoelzel AR, ed. Molecular analysis of populations: a practical approach. IRL Press, Oxford: 59-88.
• Morgante M, Hanafey M, Powell W, 2002. Microsatellites are preferentially associate with nonrepetitive DNA in plant genomes. Nature Genet 30: 194-200.
• Ordon F, Bauer E, Dehmer KJ, Graner A, Friedt W, 1995a. Identification of a RAPD-marker linked to the BaMMV/BaYMV resistance gene ym4. Barley Genet Newsl 24: 123-126.
• Ordon F, Bauer E, Graner A, Friedt W, 1995b. Marker-based selection for the BaMMV-resistance gene in barley using RAPDs. Agronomie 15: 481-485.
• Ordon F, Friedt W, Scheurer K, Pellio B, Werner K, Neuhaus G, et al. 2004. Molecular markers in breeding for virus resistance in barley. J Appl Genet 45: 145-159.
• Pellio B, Friedt W, Graner A, Ordon F, 2004. Development of PCR-based markers closely linked to rym5. J Plant Dis Prot 111: 30-38.
• Pellio B, Streng S, Bauer E, Stein N, Perovic D, Schiemann A, et al. 2005. High-resolution mapping of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-2) in barley (Hordeum vulgare ssp. vulgare L.). Theor Appl Genet 110: 283-293.
• Röder MS, Huang XQ, Ganal MW, 2004. Wheat microsatellites: potential and implications. In: Lörz H, Wenzel G, eds. Biotechnology in agriculture and forestry: molecular marker systems. Springer Verlag: 255-266.
• Rozen S, Skaletsky H, 2000. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, eds. Bioinformatics Methods and Protocols: Methods in Molecular Biology, Humana Press, Totowa: 365-386.
• Ruge B, Linz A, Pickering R, Proeseler G, Greif P, Wehling P, 2003. Mapping of Ryml4Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley. Theor Appl Genet 107: 965-971.
• Ruge-Wehling B, Linz A, Habekuß A, Wehling P, 2006. Mapping of Rym16 (Hb), the second soil-borne virus-resistance gene introgressed from Hordeum bulbosum. Theor Appl Genet 113: 867-873.
• Schiemann A, Graner A, Friedt W, Ordon F, 1996. Specificity enhancement of a RAPD marker linked to the BaMMV/BaYMV resistance gene ym4 by randomly added bases. Barley Genet Newsl 27: 63-65.
• Stein N, Perovic D, Kumlehn J, Pellio B, Stracke S, Streng S, et al. 2005. The eukaryotic translation initiation factor 4E confers multiallelic recessive Bymovirus resistance in Hordeum vulgare (L.). Plant J 42: 912-922.
• Tachida H, Iizuka M, 1992. Persistence of repeated sequences that evolve by replication slippage. Genetics 131: 471-478.
• Tatusova AT, Madden TL, 1999. Blast 2 sequences - a new tool for comparing protein and nucleotide sequences, FEMS Microbiol Lett 174: 247-250.
• Thiel T, Kota R, Grosse I, Stein N, Graner A, 2004. SNP2CAPS: a SNP and INDEL analysis tool for CAPS marker development. Nucleic Acids Res 32: e5.
• Thiel T, Michalek W, Varshney RK, Graner A, 2003. Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet 106: 41-422.
• Weber JL, 1990. Informativeness of human (dC.dA) n(dG.dT)n polymorphisms. Genomics 7: 524-530.
• Werner K, Friedt W, Laubach E, Waugh R, Ordon F, 2003. Dissection of resistance to soil-borne yellow-mosaic-inducing viruses of barley (BaMMV, BaTMV, BaYMV-2) in a complex breeders’ cross by means of SSRs and simultaneous mapping of BaYMV/BaYMV-2 resistance of var. ‘Chikurin Ibaraki 1’. Theor Appl Genet 106: 1425-1432.
• Werner K, Friedt W, Ordon F, 2005. Strategies for pyramiding resistance genes against the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-2). Mol Breed 16: 45-55.
• Wicker T, Zimmermann W, Perovic D, Paterson AH, Ganal M, Graner A, Stein N, 2005. A detailed look at 7 million years of genome evolution in a 439-kb contiguous sequence at the barley Hv-eIF4E locus: recombination, rearrangements and repeats. Plant J 41: 184-194.