Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi

• Autorzy: Kruszewska J. S. , Perlinska-Lenart U. , Gorka-Niec W. , Orlowski J. , Zembek P. , Palamarczyk G.
• Języki publikacji: EN

Abstrakty

EN

Due to its natural properties, Trichoderma reesei is commonly used in industry-scale production of secretory proteins. Since almost all secreted proteins are O-glycosylated, modulation of the activity of enzymes of the O-glycosylation pathway are likely to affect protein production and secretion or change the glycosylation pattern of the secreted proteins, altering their stability and biological activity. Understanding how the activation of different components of the O-glycosylation pathway influences the glycosylation pattern of proteins and their production and secretion could help in elucidating the mechanism of the regulation of these processes and should facilitate creation of engineered microorganisms producing high amounts of useful proteins. In this review we focus on data concerning Trichoderma, but also present some background information allowing comparison with other fungal species.

Słowa kluczowe

PL

fungi; glycosylation; metabolic engineering; protein secretion; alteration; Trichoderma; biotechnology; protein production

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 3
• Opis fizyczny: p.447-456,fig.,ref.

Twórcy

autor

Kruszewska J. S.

• Polish Academy of Sciences, Adolfa Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Perlinska-Lenart U.

autor

Gorka-Niec W.

autor

Orlowski J.

autor

Zembek P.

autor

Palamarczyk G.

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