Use of a microbore ODS column for the separation of paprika [Capsicum annuum] pigments by high performance liquid chromatography

• Autorzy: Cserhati T. , Forgacs E. , Morais H. , Ramos C.
• Języki publikacji: EN

Abstrakty

EN

The colour pigments of paprika (Capsicum annuun) were separated by reversed-phase high-performance liquid chromatography (HPLC) using normal (4 mm I.D.) and microbore (2 mm I.D.) C18 silica columns and gradient elution (water-acetonitrile-methanol). The relative standard deviation of retention times (lower than 1.5%) and that of peak areas (3-5%) were similar for both columns proving the good stability of the HPLC system. The high standard deviation of peak areas may be due to the presence of overlapping peaks. The number of separated pigment fractions was considerably higher on microbore (75) than on normal ODS column (34) proving the superiority of microbore column.

Słowa kluczowe

EN

colour pigment; Capsicum annuum; pepper; high performance liquid chromatography

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2002
• Tom: 11/52
• Numer: 1
• Opis fizyczny: p.11-13,fig.

Twórcy

autor

Cserhati T.

• Hungarian Academy of Sciences, P.O.Box 17, 1525 Budapest, Hungary

autor

Forgacs E.

autor

Morais H.

autor

Ramos C.