Zdolnosc akceptacji bakteriofagow warunkujacych produkcje verotoksyn przez paleczki z rodziny Enterobacteriaceae

• Autorzy: Sobieszczanska B. , Gryko R.
• Języki publikacji: PL

Abstrakty

PL

Przekazywanie bakteriofagów niosących geny dla verotoksyn (Shiga-like toksyn) - stx-1 i stx-2, między pałeczkami jelitowymi odgrywa kluczową rolę w rozprzestrzenianiu się szczepów zdolnych do produkcji verotoksyn. Bakteriofagi te, w przeciwieństwie do dzikich szczepów pałeczek jelitowych, są łatwo akceptowane przez laboratoryjny szczep E. coli C600. Spośród 125 dzikich szczepów pałeczek z rodziny Enterobacteriaceae, głównie E. coli, żaden in vitro nie akceptował bakteriofagów warunkujących wytwarzanie verotoksyn.

EN

Lysogenised verotoxigenic strains are the source of structural genes of verocytotoxins (stx-1 and stx-2) for the others intestinal bacili. The aim of the study was to estimate the ability of transfer of bacteriophages induced with UV irradiation from reference verotoxigenic strains of E. coli O157:H7 (CB571 and EDL933) into 125 wild-strains of bacili of Enterobacteriaceae family. None of tested recipient strains showed the production of cytotoxin on Vero and HeLa cell lines, what was acknowledged as the lack of stx genes. Contrary to the laboratory strain of E. coli C600 none of 125 tested recipient strains accepted the phages. Obtained lysogenised laboratory strains of E. coli C600/CB571 and E. coli C600/EDL933, besides of the ability to produce verotoxins (with the presence of stx-1 and stx-2 genes), did not differ phenotypically and genotypically from parent strain of E. coli C600. The estimation of the ability to transfer of phages carried stx-1 and/or stx-2 genes was impossible because of too small number of tested wild strain of bacili or because of really low frequency of acceptation of phages by wild strains of intestinal bacili.

Słowa kluczowe

PL

bakteriofagi; mikrobiologia lekarska; Escherichia coli; verotoksyny; Enterobacteriaceae; produkcja; bakterie; zroznicowanie bakterii; geny

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 3
• Opis fizyczny: s.269-276,fot.,tab.,bibliogr.

Twórcy

autor

Sobieszczanska B.

• Akademia Medyczna, Wroclaw

autor

Gryko R.

Bibliografia

• 1. Acheson DW, Reidl J, Zhang X i inni. In vivo transduction with Shiga toxin 1 – encoding phage. Infect Immun 1998; 66: 4496-98.
• 2. Beutin L, Montenegro M, Ørskov I i inni. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J Clin Microbiol 1989; 11: 2559-64.
• 3. Beutin L, Ørskov I, Ørskov F i inni. Clonal diversity and virulence factors in strains of E. coli of the classic enteropathogenic serogroup O114. J Infect Dis 1990; 162: 1329-34.
• 4. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res. 1979; 7: 1513-23.
• 5. Chinyu S, Brandt LJ. Escherichia coli O157:H7 infection in humans. Ann Intern Med 1995; 123: 698 -714.
• 6. Evans DJ, Evans DG, Young LS, Pitt J. Hemagglutination typing of Escherichia coli: Definition of seven hemagglutination types. J Clin Microbiol 1980; 12: 235-42.
• 7. Forestier CH, Meyer M, Favre-Bonte S i inni. Enteroadherent Escherichia coli and diarrhea in children: a prospective case-control study. J Clin Microbiol 1996; 34, 12: 2897-2903.
• 8. Haque QM, Sugiyama A, Iwade I i inni. Diarrheal and enviromental isolates of Aeromonas spp. produce a toxin similar to Shiga-like toxin 1. Curr Microbiol 1996; 32: 239-45.
• 9. Karch H, Meyer T, Rüssmann H, Heesemann J. Frequent loss of Shiga-like toxin genes in clinical isolates at Escherichia coli upon subcultivation. Infect Immun 1992; 60, 8: 3464-67.
• 10. Lindquist R. Preparation of PCR samples from food by a rapid and single centrifugation technique evaluated by detection of Escherichia coli O157:H7. Int J Food Microbiol 1997; 37: 73-82.
• 11. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning. A laboratory manual. NewYork Cold Spring Harbor Laboratory 1982, str. 63-73.
• 12. Pierard D, Stevens D, Morian L i inni. Isolation and virulence factors of verotoxin-producing Escherichia coli in human stool samples. Clin Microbiol Infect 1997; 3, 5: 531-39.
• 13. Schmidt HB, Kohler A, Bielaszewska M, Karch H. The role of stx-encoding bacteriophages in pathogenicity and virulence of Shiga toxin-producing Escherichia coli (STEC). Pathogenicity and Virulence of Verocytotoxigenic E. coli-Proceedings of a meeting hosted by the University of Liege, Belgium 1999, Editors: Duffy G, Garvey P, Coia J, Wateson Y, McDowell DA. Published by Teagasc, The National Food Centre, Dublin 15, Ireland, str. 11-17.
• 14. Scotland SM, Smith HR, Willshaw GA, Rowe B. Vero cytotoxin production in strains of Escherichia coli is determined by genes carried on bacteriophage. Lancet 1983; 2: 216.
• 15. Smith HR Green WP, Parsell Z. Vero cell toxin in Escherichia coli and related bacteria: transfer by phage and conjugation and toxin action in laboratory animals, chickens and pigs. J Gen Microbiol 1983; 129: 3121-37.
• 16. Tschape H, Pragner R, Streckel W i inni. Outbreak of cases of hemolytic uremic syndromes and gastroenteritis in a nursery school-verotoxinogenic Citrobacter freundii as a causative agent. Program and Abstracts of 2nd International Symposium and Workshop on ‘Verocytotoxin (Shiga-like toxin)-producing Escherichia coli’ Bergamo, Italy 1994; str: 22.
• 17. Tóth I. Phages and their role in virulence of bacteria. Pathogenicity and Virulence of Verocytotoxigenic E. coii-Proceedings of a meeting hosted by the University of Liege, Belgium 1999, Editors: Duffy G, Garvey P, Coia J, Wateson Y, McDowell DA. Published by Teagasc, The National Food Centre, Dublin 15, Ireland, str. 1-10.
• 18. Tzipori S, Karch H, Wachsmuth KJ i inni. Role of a 60-Megadalton plasmid and Shiga-like toxins in the pathogenesis of infection caused by enterohemorrhagic Escherichia coli O157:H7 in gnotobiotic piglets. Infect Immun 1987; 55, 12: 3117-25.