Melatonin in human volunteers: focus on nasal lavage level and its free radical scavenging properties with possibility to apply in food research - a short report

• Autorzy: Zielinski H. , Mudway I. , Lewczuk B. , Przybylska-Gornowicz B. , Kelly F.J.

Warianty tytułu

PL

Poziom melatoniny w plynie uzyskanym z plukania komory nosowej oraz jego aktywnosc przeciwutleniajaca - krotki komunikat

• Języki publikacji: EN

Abstrakty

EN

The present paper is the first to report the results of the work carried out on daytime melatonin levels in paired plasma and nasal lavage fluid (NAL) samples obtained from healthy adult subjects. Lavage fluid was collected from 25 volunteers using a modified Foley catheter. Blood plasma was collected using normal procedure. The immunoreactive melatonin was present in all plasma samples and in 80% of the investigated NAL samples. Blood plasma melatonin level ranged from 0.98 pg/mL to 34.97 pg/mL however the level found in NAL was lower and ranged from 0.46 to 2.42 pg/ mL There was no correlation between paired plasma and nasal lavage melatonin concentration (r=0.02). Moreover, the superoxide dismutase activity (SOD) in NAL samples was determined and then the correlation coefficient between the melatonin concentration and EC-SOD activity was r= 0.18. The peroxyl radical trapping activity of 1 μmol/L melatonin was equivalent to 3 μmol/L Trolox. It has been concluded that nasal lavage fluid melatonin has limited utility in the field of melatonin research.

PL

W pracy po raz pierwszy przedstawiono zawartość melatoniny w osoczu krwi i płynie uzyskanym z płukania komory nosowej zdrowych dorosłych 25 ochotników. Próby do badań pobierano w dzień według przyjętych procedur. Obecność melatoniny stwierdzono we wszystkich próbach osocza oraz w 20 próbach płynu uzyskanego z płukania komory nosowej. Poziom melatoniny w osoczu był w przedziale od 0,98 pg/mL do 34,97 pg/mL (tab. 1) natomiast w uzyskanym płynie zmieniał się od 0,46 do 2,42 pg/mL (tab. 2). Nie stwierdzono korelacji pomiędzy zawartością melatoniny w osoczu i płynie z płukania komory nosowej od indywidualnych ochotników (r=0,02). Zbadano także aktywność dysmutazy ponadtlenkowej (SOD) w płynie i stwierdzono jej słabą korelację z zawartością w nim melatoniny (r= 0,18) (rys. 3). Prześledzono ponadto zdolność melatoniny do wymiatania rodników nadtlenkowych. Wykazano, że zdolność 1 μmola melatoniny do wymiatania rodników nadtlenkowych była równoważna tej wykazywanej przez 3 μmole Troloksu (rys. 2). Uznano, że płyn uzyskiwany z płukania komory nosowej zdrowych ochotników nie stanowi ważnego materiału biologicznego w badaniach nad występowaniem i właściwościami melatoniny.

Słowa kluczowe

EN

man; volunteer; blood plasma; nasal lavage fluid; melatonin; superoxide dismutase activity; food research; healthy man; melatonin concentration

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 2
• Opis fizyczny: p.215-219,fig.,ref.

Twórcy

autor

Zielinski H.

• Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Mudway I.

autor

Lewczuk B.

autor

Przybylska-Gornowicz B.

autor

Kelly F.J.

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