DNA fingerprinting analysis of rabbits from lines divergently selected for high and low open-field activity

• Autorzy: Sacharczuk M , Jezierski T. , Daniewski W. , Gorecka A. , Parada R. , Swiergiel A.H. , Jaszczak K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to analyse whether a successful divergent selection for open-field activity (OFA) in rabbits has resulted in differences in DNA fingerprinting pattern and genetic parametres of diversity. We also scanned DNA fingerprinting profiles for searching minisatellite alleles potentially linked to genes determining the trait under selection. Rabbits derived from the 8th generation of the lines selected for high (H) or low (L) locomotor OFA were profiled for DNA fingerprinting using HinfI enzyme and 33.6 multilocus probe. The H and L lines represent different, i.e. active and passive coping strategies in a novel situation.Selection for H or L locomotor OFA did not affect significantly the molecular parametres of genetic diversity. However, the analysis of band patterns for individual and pooled DNA fingerprints revealed a specific band for the L line at 15 kbp. No specific bands for the H line were detected. The results presented provide evidence of a possible linkage between minisatellites and OFA in rabbits and demonstrate that studies on the H and L lines may give rise to a new strategy in animal breeding and selection for traits related to animal welfare (locomotion and fear-related emotional behavior) and to the study of genetic background of hyperactivity disorders, e.g. ADHD.

PL

Celem badań było określenie, czy selekcja królików na aktywność w otwartym polu spowodowała zróżnicowanie we wzorze DNA fingerprinting, a także czy efektem tej selekcji jest zmiana genetycznych parametrów zmienności na poziomie molekularnym. Ponadto wzory DNA fingerprinting analizowano pod względem obecności specyficznych alleli minisatelitarnych, które potencjalnie mogą być sprzężone z genami determinującymi selekcjonowaną cechę. Profilowanie DNA fingerprinting przeprowadzono na ósmym pokoleniu królików selekcjonowanych na wysoką (H) i niską (L) aktywność w otwartym polu, z użyciem enzymu HinfI i wielopozycyjnej sondy 33.6. Rozbieżna selekcja nie spowodowała znaczącej zmiany w genetycznych parametrach zmienności. Jednakże analiza indywidualnych i reprezentatywnych wzorów prążkowych pozwoliła na identyfikację w linii L specyficznego allelu minisatelitarnego o długości 15 tysięcy par zasad. Nie wykryto specyficznych alleli u osobników linii H.

Słowa kluczowe

EN

selection; open field activity; minisatellite; DNA fingerprinting; animal breeding; molecular marker; genetic diversity; rabbit

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2005
• Tom: 23
• Numer: 2
• Opis fizyczny: p.107-117,fig.,ref.

Twórcy

autor

Sacharczuk M

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Jezierski T.

autor

Daniewski W.

autor

Gorecka A.

autor

Parada R.

autor

Swiergiel A.H.

autor

Jaszczak K.

Bibliografia

• 1. BENJAMIN J., LI L., PATTERSON C., GREENBERG B.D., MURPHY D.L., HAMER D.H.,1996 – Population and familial associations between D4 dopamine receptor gene and measures of novel seeking. Nature Genetics 12, 81-84.
• 2. BOISSY A., 1995 – Fear and fearfulness in animals. The Quarterly Review of Biology 70, 165-191.
• 3. CAROLA V., D’OLIMPIO F., BRUNAMONTI E., MANGIA F., RENZI P., 2002 – Evaluation of the elevated plus-maze and open-field test for the assessment of anxiety-related behaviour in inbred mice. Behavioural Brain Research 134, 49-57.
• 4. CHOLERIS E., THOMAS A.W., KAVALIERS M., PRATO F.S., 2001 – A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neuroscience and Biobehavioral Reviews 25, 235-260.
• 5. COOLS A.R., GINGRAS M.A., 1998 – Nijmegen high and low responders to novelty: a new tool in the search after the neurobiology of drug abuse liability. Pharmacology, Biochemistry and Behavior 60, 151-159.
• 6. CRAWLEY J.N., BELKNAP J.K., COLLINS A., CRABBE J.C., FRANKEL W., HENDERSON N., HITZEMANN R.J., MAXSON S.C., MINER L.L., SILVA A.J., WEHNER J.M., WYNSHAWBORIS A., PAYLOR R., 1997 – Behavioral phenotypes of inbred mouse strains: implications and recommendations for molecular studies. Psychopharmacology (Berl). 132, 107-124.
• 7. DANIEWSKI W., JEZIERSKI T., 2003 – Effectiveness of divergent selection for open-field activity in rabbits and correlated response for body weight and fertility. Behavior Genetics 33, 337-345.
• 8. DENENBERG V.H., DESANTIS D., WAITE S., THOMAN E.B., 1977 – The effects of handling in infancy on behavioral states in the rabbit. Physiology & Behavior 18, 553-557.
• 9. DE PASSILLE A.M., RUSHEN J., MARTIN F., 1995 – Interpreting the behaviour of calves in an open-field test: a factor analysis. Applied Animal Behaviour Science 45, 201-213.
• 10. DESAUTES C., BIDANEL J.P., MILAN D., IANNUCCELLI N., AMIGUES Y., BOURGEOIS F., CARITEZ J.C., RENARD C., CHEVALET C., MORMEDE P., 2002 – Genetic linkage mapping of quantitative trait loci for behavioural and neuroendocrine stress response trait in pigs. Journal of Animal Science 80, 2276-2285.
• 11. FLINT J., 2003 – Analysis of Quantitative Trait Loci that influence animal behavior. Journal of Neurobiology 54, 46-77.
• 12. FLINT J., DEFRIES J.C., HENDERSON N.D., 2004 – Little epistasis for anxiety-related measures in the DeFries strains of laboratory mice. Mammalian Genome 15, 77-82.
• 13. GERSHENFELD H.K., NEUMANN P.E., MATHIS C., CRAWLEY J.N., LI X., PAUL S.M., 1997 – Mapping quantitative trait loci for open-field behavior in mice. Behavior Genetics 27, 201-210.
• 14. HALL D.C., 1934 – Emotional behavior in the rat. I. Defecation and urination as individual differences in emotionality. Journal of Comparative and Physiological Psychology 18, 385-403.
• 15. HENDERSON N., TURRI M.G., DEFRIES J.C., FLINT J., 2004 – QTL analysis of multiple behavioral measures of anxiety in mice. Behavior Genetics 34, 267-293.
• 16. HIRSJARVI P., VALIAHO T., 1995 – Effects of gentling on open-field behaviour of Wistar rats in fear-evoking test situation. Laboratory Animals 29, 380-384.
• 17. JEFFREYS A.J., WILSON V., THEIN S.L., 1985 – Hypervariable ,,minisatellite” regions in human DNA. Nature 314, 67-73.
• 18. KERSTEN A.M.P., MEIJSSER F.M., METZ J.H.M., 1989 – Effects of early handling on later open field behaviour in rabbits. Applied Animal Behaviour Science 24, 157-167.
• 19. KUHNLEIN U., DAWE Y., ZADWORNY D., GAVORA J.S., 1989 – DNA fingerprinting: a tool for determining genetic distances between strains of poultry. Theoretical and Applied Genetics 77,669-672.
• 20. LESCH K.P., BENGEL D., HEILS A., SABOL S.Z., GREENBERG B.D., PETRI S., BENJAMIN J., MULLER C.R., HAMER D.H., MURPHY D.L., 1996 – Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region. Science 274, 1527-1531.
• 21. LYNCH M., 1990 – The similarity index and DNA fingerprinting. Molecular Biology and Evolution 7, 478-484.
• 22. MECHAN A.O., MORAN P.M., ELLIOTT M., YOUNG A.J., JOSEPH M.H., GREEN R., 2002 – A comparison between Dark Agouti and Sprague-Dawley rats in their behaviour on the elevated plusmaze, open-field apparatus and activity meters, and their response to diazepam. Psychopharmacology (Berl).159, 188-195.
• 23. MIGNON-GRASTEAU S., ROUSSOT O., DELABY C., FAURE J.M., MILLS A., LETERRIER C., GUEMENE D., CONSTANTIN P., MILLS M., LEPAPE C., BEAUMONT C., 2003 – Facrorial correspondence analysis of fear related behaviour traits in Japanese quail. Behavioural Processes 61,69-75.
• 24. MORMEDE P., MONEVA E., BRUNEVAL C., CHAOULOFF F., MOISAN M.P., 2002 – Markerassisted selection of neuro-behavioural trait related to behavioural trait related to behavioural inhibition in the SHR strain, an animal model of ADHD. Genes, Brain and Behavior 1, 111-116.
• 25. PRUT L., BELZUNG C., 2003 – The open-field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. European Journal of Pharmacology 463, 3-33.
• 26. RAFAY J., FL’AK P., 1998 – Croosbreeding parameters of rabbits motion under open field conditions.World Rabbit Science 7, 9-14.
• 27. RAMOS A., BERTON O., MORMEDE P., CHAOULOFF F., 1997 – A multiple-test study of anxiety-related behaviours in six inbred rat strains. Behavioural Brain Research 85, 57-69.
• 28. RUSSELL R.J., FESTING M.F., DEENY A.A., PETERS A.G., 1993 – DNA fingerprinting for genetic monitoring of inbred laboratory rats and mice. Laboratory Animal Science 43, 460-465.
• 29. SAS Institute, 1990 – SAS/STAT Users’ Guide, 4th ed., Cary, NC.
• 30. SCHMITT V., HIEMKE C., 1998 – Strain differnces in open-field and elevated plus-maze behavior of rats without and with pretest handling. Pharmacology, Biochemistry and Behavior 59, 807-811.
• 31. SCHLOTTERER C., 2004 – The evolution of molecular markers—just a matter of fashion? Naure Reviews. Genetics 5, 63-69.
• 32. STAM R., CROISET G., AKKERMANS L.M.A., WIEGANT V.M., 1997 – Behavioural and intestinal responses to novelty in rats selected for diverging reactivity in the open field test. Behavioural Brain Research 88, 231-238.
• 33. STEPHENS J.C., GILBERT D.A., YUHKI N., O’BRIEN S.J., 1992 – Estimation of heterozygosity for single probe multilocus DNA fingerprints. Molecular Biology and Evolution 9, 729-743.
• 34. SUDO T., TSUJI S., MANNEN H., GOTO N., UMEZAWA H., MIZUTANI M., YAZAWA H.,SHIOMI M., 1993 – Application of DNA fingerprinting to investigation of genetic relationships between laboratory rabbit strains. Jikken Dobutsu 42, 337-342.
• 35. TURRI M.G., HENDERSON N.D., DEFRIES J.C., FLINT J., 2001 – Quantitative trait locus mapping in laboratory mice derived from a replicated selection experiment for open-field activity. Genetics 158, 1217-1226.
• 36. TURRI M.G., TALBOT C.J., RADCLIFFE R.A., WEHNER J.M., FLINT J., 1999 – High-resolution mapping of quantitative trait loci for emotionality in selected strains of mice. Mammalian Genome 10, 1098-1101.
• 37. WALSH R.N., CUMMINS R.A., 1975 – Mechanisms mediating the production of environmentally induced brain changes. Psychological Bulletin 82, 986-1000.
• 38. WETTON J.H., CARTER R.E., PARKIN D.T., WALTERS D., 1987 – Demographic study of a wild sparrow population by DNA fingerprinting. Nature 327, 147-152.
• 39. ZELNIK J., KOLATAJ A., RAFAY J., PARKANY V., BULLA J., FL’AK P., TOKARSKI J., 1990 – Motor activity of rabbits of various genetics groups in the open field. Journal of Animal Breeding and Genetics. 107, 465-469.