Analysis of Polish isolates of Borrelia burgdorferi by amplification of rrf [5S]-rrl [23S] intergenic spacer

• Autorzy: Mizak B , Krol J.
• Języki publikacji: EN

Abstrakty

EN

Recently, on the basis of molecular analysis, the number of B. burgdorferi sensu lato genomic groups has been extended to eight. The aim of our studies was to identify B. burgdorferi sensu lato strains isolated from ticks and dogs in Poland on the basis of fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. The analysis of amplicon sizes revealed that two Polish tick-originated strains: 236/2 and KL43 represent B. lusitaniae genogroup; strain KL5 - B. afzelii; strains KL44, KL93 and KL1 - B. burgdorferi sensu stricto. Strain KL3 and KL4 may represent a separate genogroup of B. burgdorferi. Three strains isolated from dogs (P3, P4 and P6) with the amplicon sizes of 267 bp, 262 bp and 262 bp respectively, form a coherent cluster.

Słowa kluczowe

EN

rRNA gene organization; Poland; rrfA gene; gene encoding; Lyme disease; molecular analysis; DNA hybridization; rrfB gene; polymerase chain reaction; Borrelia burgdorferi

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 2
• Opis fizyczny: p.147-154,fig.

Twórcy

autor

Mizak B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Krol J.

Bibliografia

• 1. Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R., Lathigra R., White O., Ketchum K.A., Dodson R., Hickey E.K., Gwinn M., Dougherty B., Tomb J.F., Fleischmann R.D., Richardson D., Peterson J., Kerlavage A.R., Quackenbush J., Salzberg S., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J., Weidman J., Utterback T., Watthey L., McDonald L., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Roberts K., Hatch B., Smitch H.O., Venter J.C.: Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature, 1997, 390, 580-586.
• 2. Ojaimi C., Davidson B.E., Girons I.S., Old I.G.: Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii. Microbiology, 1994, 140, 2931-2940.
• 3. Postic D., Assous M.V., Grimont P.A.D., Baranton G.: Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment lenght polymorphism of rrf (5S) rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol., 1994, 44, 743-752.
• 4. Postic D., Korenberg E., Gorelova N., Kovalevski Y.V., Bellenger E., Baranton G.: Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incidence of mixed isolates. Res. Microbiol., 1997, 148, 691-702.
• 5. Postic D., Ras N.M., Lane R.S., Hendson M., Baranton G.: Expanded diversity among Californian Borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127). J. Clinic. Microbiol., 1998, 36, 3497-3504.
• 6. Rijpkema S.G.T., Molkenboer M.J.C.H., Schouls L.M., Jongejan F., Schellekens J.F.P.: Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch lxodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol., 1995, 33, 3091-3095.
• 7. Saint Girons L, Old I.G., Davidson B.E.: Molecular biology of the Borrelia burgdorferi, bacteria with linear replicons. Microbiology, 1994, 140, 1803-1816.
• 8. Saint GironsI., Gern L., Gray J.S., Guy E.C., Korenberg E., Nuttall P.A., Rijpkema S.G.T., Schonberg A., Stanek G., Postic D.: Identification of Borrelia burgdorferi sensu lato species in Europe. Zent. BI. Bacteriol., 1998, 287, 190-195.
• 9. Siński E., Karbowiak G., Marciniak T., Stelmaszyk Z., Rijpkema S.: Kleszcze Ixodes ricinus w szerzeniu się zakażeń Borrelia burgdorferi sensu lato. Międzynarodowa Konferencja Naukowa nt: "Borelioza z Lyme", Warszawa 5 listopada 1996. Materiały konferencyjne pp. 116-121.
• 10. Wang G., Dam A.P., Le Fleche A., Postic D., Peter O., Baranton G., Boer R., Spanjaard L., Dankert J.: Genetic and phenotypic analysis of Borrelia valaisiana sp. nov. (Borrelia genomic groups VS116 and M19). Int. J. Syst. Bacteriol. 1997, 47, 926-932.