Effect of different doses of deoxynivalenol on metabolism in broiler chickens

• Autorzy: Faixova Z , Faix S. , Borutova R. , Leng L.
• Języki publikacji: EN

Abstrakty

EN

The experiment was conducted to investigate the effect of different doses of deoxynivalenol on plasma indices of broiler chickens. Forty-two one-day-old male broiler chicks were fed 1 of 3 diets containing deoxynivalenol (DON) for 42 d. The diets included: (1) control (0.2 ppm of deoxynivalenol), (2) low level of deoxynivalenol (1 ppm of DON), and (3) high level of deoxynivalenol (3 ppm of DON). Then, all the birds were sacrificed and blood samples for biochemical analyses were collected. The mycotoxin doses in diets were verified using gas chromatography-mass spectrometry. The administration of 1 ppm of DON altered total protein, triglycerides, free glycerol, and potassium levels. Dietary addition of 3 ppm of DON resulted in altered calcium, potassium, total protein, triglycerides, along with free glycerol levels, and aspartate aminotransferase activity. No biochemical parameter, however, responded to increased DON concentration in the diet. The feeding of DON-containing diets did not significantly alter plasma chloride, cholesterol, and albumin levels or aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase activities. It was concluded that both levels of deoxynivalenol in the diets tested significantly affected protein and lipid metabolism in broiler chicks.

Słowa kluczowe

EN

mycotoxin; metabolism; broiler chicken; veterinary medicine; deoxynivalenol; blood biochemistry; chicken; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 3
• Opis fizyczny: p.421-424,ref.

Twórcy

autor

Faixova Z

• University of Veterinary Medicine in Kosice, 041 81 Kosice, Slovakia

autor

Faix S.

autor

Borutova R.

autor

Leng L.

Bibliografia

• 1. Accensi F., Pinton P., Gallu P., Abella-Bourges N., Guelfi J.F., Grosjean F., Oswald I.P.: Ingestion of low doses of deoxynivalenol does not affect hematological, biochemical, or immune responses of piglets. J Anim Sci 2006, 84, 1935-1942.
• 2. Bergsjø B., Langseth W., Nafstad I., Jansen J.H., Larsen H.J.S.: The effects of naturally deoxynivalenol-contaminated oats on the clinical conditions, blood parameters, performance, and carcass composition of growing pigs. Vet Res Commun 1993, 17, 283-294.
• 3. Dänicke S., Valenta H., Klobasa F., Döll S., Ganter M., Flachowsky G.: Effect of graded levels of Fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrient digestibility, deoxynivalenol balance and clinical serum characteristics. Arch Anim Nutr 2004, 58, 1-17.
• 4. Deacon A.C, Dawson P.J.: Enzymatic assay of total cholesterol involving chemical or enzymatic hydrolysis-a comparison of methods. Clin Chem 1979, 25, 936-938.
• 5. Doll S., Dänicke S., Valenta H., Flachowsky G.: In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone. Arch Anim Nutr 2004, 58, 311-324.
• 6. Doumas B.T., Bayse D.D., Carter R.J., Peters T. Jr., Schaffer R.: A candidate reference method for determination of total protein in serum. I. Development and validation. Clin Chem 1981, 27, 1642-1650.
• 7. Farnworth E.R., Hamilton R.M., Thompson B.K., Trenholm H.L.: Liver lipid levels in White Leghorn hens fed diets that contained wheat contaminated by deoxynivalenol (vomitoxin). Poult Sci 1983, 62, 832-836.
• 8. Gouze M.E., Laffitte J., Rouimi P., Loiseau N., Oswald I.P., Galtier P.: Effect of various doses of deoxynivalenol on liver xenobiotic metabolizing enzymes in mice. Food Chem Toxicol 2006, 44, 476-483.
• 9. Hilmann G., Beyer G.: Rapid determination of serum potassium by turbidity measurement with kalignost after protein precipitation. Z Klin Chem Klin Biochem 1967, 2, 93-94.
• 10. Huff W.E., Kubena L.F., Harvey R.B., Hagler W.M., Swanson S.P., Phillips T.D., Creger C.R.: Individual and combined effects of aflatoxin and deoxynivalenol (DON, vomitoxin) in broiler chickens. Poult Sci 1986, 65, 1291-1298.
• 11. Chromý V., Zahradnicek L., Vozníček J.: Use of N-methyl D-glucamine as buffer in the determination of serum alkaline phosphatase activity. Clin Chem 1981, 10, 1729-1732.
• 12. Koditschek L.K., Umbreit W.W.: α-Glycerophosphate oxidase in Streptococcus faecium F 24. J. Bacteriol 1969, 98, 1063-1068.
• 13. Kubena L.F., Edrington T.S., Harvey R.B., Buckley S.A., Phillips T.D., Rottinghans G.E., Casper H.H.: Individual and combined effects of fuminisin B₁ present in Fusarium moniliforme culture material and T-2 toxin or deoxynivalenol on broiler chicks. Poult Sci 1997, 76, 1230-1247.
• 14. Kubena L.F., Harvey R.B., Corrier D.E., Huff W.E., Phillips T.D.: Effect of feeding deoxynivalenol (DON, vomitoxin) contaminated wheat to female White Leghorn chickens from day old through egg production. Poult Sci 1987, 66, 1612-1618.
• 15. Kubena L.F., Huff W.F., Harvey R.B., Corrier D.E., Phillips T.D.: Influence of ochratoxin A and deoxynivalenol on growing broiler chicks. Poult Sci 1988, 67, 253-260.
• 16. Kubena L.F., Swanson S.P., Harvey R.B., Fletcher O.J., Rowe L.D., Phillips T.D.: Effect of feeding deoxynivalenol (vomitoxin) contaminated wheat to growing chicks. Poult Sci 1985, 64, 1649-1655.
• 17. Kuffer H., Richterich R., Kraft R., Peheim E., Colombo J.A.P.: The determination of chloride in plasma and serum (mercuty (II)-thiocyanate method) with the Greiner Electronic Selective Analyzer GSA II. Z Klin Chem Klin Biochem 1975, 5, 203-211.
• 18. Mann C.K., Yoe J.H.: Spectrophotometric determination of magnesium with sodium l-azo-2 hydroxy-3-(2,4-dimethylcarboxanilido) naphthalene-1'-(2- hydroxybenzene). Anal Clin Act 1957, 16, 155-160.
• 19. Maresca M., Mahfoud R., Garmy N., Fantini J.: The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells. J Nutr 2002, 123, 2723-2731.
• 20. Mezes M., Barta M., Nagy G.: Comparative investigation on the effect of T-2 mycotoxin on lipid peroxidation and antioxidant status in different poultry species. Res Vet Sci 1999, 66, 19-23.
• 21. Mikami O., Yamamoto S., Yamanaka N., Nakajima Y.: Porcine hepatocytes apoptosis and reduction of albumin secretion induced by deoxynivalenol. Toxicol 2004, 15, 241-249.
• 22. Pinnel A.E., Northam B.E.: New automated dye-binding method for serum albumin determination with bromcresol purple. Clin Chem 1978, 24, 80-86.
• 23. Placinta C.M., D'Melio J.P.F., Mac Donald A.M.C.: A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim Feed Sci Technol 1999, 78, 21-37.
• 24. Raymond S.L., Smith T.K., Swamy H.V.L.N.: Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum chemistry, and hematology of horses, and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J Anim Sci 2003, 81, 2123-2130.
• 25. Ray Sarkar B.C., Chauchan U.S.P.: Anew method for determination of microquantities of calcium in biological material. Anal Biochem 1967, 20, 155-166.
• 26. Reitman S., Frankel S.: A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Amer J Clin Pathol 1957, 28, 56-63.
• 27. Rotter B.A., Prelusky D.B., Pestka J.J.: Toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health, 1996, 48, 1-34.
• 28. Sergeev I.N., Piliia N.M., Kuzmina E.E., Avreneva L.I., Kravchenko L.V., Spirichev V.B., Tutel'ian V.A.: Calcium and vitamin D metabolism and enzymes of xenobiotic metabolism during chronic action of mycotoxins. Vopr Pitan 1990, 5, 25-30.
• 29. Swamy H.V.L.N., Smith T.K., Karrow N.A., Boermans H.J.: Effect of feeding blends of grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens. Poult Sci 2004, 83, 533-543.
• 30. Wroblewski F., La Due J.: Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med 1955, 90, 210-213.