Plasminogen activator inhibitor 1 [PAI-1] 1334G-A genetic polymorphism in colorectal cancer

• Autorzy: Smolarz B. , Romanowicz-Makowska H. , Kulig A.
• Języki publikacji: EN

Abstrakty

EN

Plasminogen activator inhibitor 1 (PAI-1) content in colorectal cancer tissue ex­tracts may be of strong prognostic value: high levels of PAI-1 in tumours predict poor prognosis. The gene encoding PAI-1 is highly polymorphic and PAI-1 gene variability could contribute to the level of PAI-1 biosynthesis. In the present work the distribu­tion of genotypes and frequency of alleles of the 1334G/A polymorphism in 92 subjects with colorectal cancer in samples of cancer tissue and distant mucosa samples as well as in blood were investigated. Blood samples age matched healthy individuals (n = 110) served as control. The 1334G/A polymorphism was determined by PCR ampli­fication using allele specific primers. No differences in the genotype distributions and allele frequencies between blood, distant mucosa samples and cancer tissue were de­tected. However, the distribution of the genotypes of the 1334G/A polymorphism in patients differed significantly (P < 0.05) from those predicted by the Hardy-Weinberg equilibrium. There were significant differences in the frequencies of alleles between the colorectal cancer subjects and controls (P < 0.05). The results support the hypothe­sis that the 1334G/A polymorphism may be associated with the incidence of colorectal cancer.

Słowa kluczowe

EN

tumour cell; lymphatic vessel; extracellular matrix; blood stream; metastasis; genetic polymorphism; plasminogen activator inhibitor; polymorphism; malignant tumour; polymerase chain reaction; colorectal cancer; cancer; prognostic marker; tissue

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 2
• Opis fizyczny: p.489-495,fig.

Twórcy

autor

Smolarz B.

• Institute of Polish Mother's Memorial Hospital, Rzgowska 281-289, 93-338 Lodz, Poland

autor

Romanowicz-Makowska H.

autor

Kulig A.

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