Lipoarabinomannan as a regulator of the monocyte apoptotic response to Mycobacterium bovis BCG Danish strain 1331 infection

• Autorzy: Krzyzowska M , Schollenberger A. , Pawlowski A. , Hamasur B. , Winnicka A. , Augustynowicz-Kopec E. , Niemialtowski M.
• Języki publikacji: EN

Abstrakty

EN

The mannosylated lipoarabinomannan (ManLAM) from mycobacterial species possesses strong immunomodulatory effects. Here we examined the ability of Mycobacterium tuberculosis ManLAM to interfere with the apoptotic response of mouse monocyte cell line, RAW 264.7 infected with Mycobacterium bovis BCG Danish strain. Incubation of BCG-infected monocytes with ManLAM decreased production of NO and the numbers of apoptotic cells which synergized with the polarization of mitochondrial membrane. Activities of caspase-1, -3, -8 and 9 followed pattern of apoptosis suppression by ManLAM, except for caspase-1, which showed no significant change in activity. ManLAM also stabilized anti-apoptotic ratio of bcl-2/bax expression in BCG-infected cells and blocked activation of Fas/FasL-induced pathway of apoptosis. Thus, ManLAM, apart from blocking mitochondrial pathway of apoptosis, may induce several other pathways regulating apoptotic response in BCG-infected mouse monocytes.

Słowa kluczowe

EN

Danish strain; cell line; infection; Mycobacterium bovis; Mycobacterium tuberculosis; monocyte; apoptosis; monocyte apoptotic response; lipoarabinomannan

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2007
• Tom: 56
• Numer: 2
• Opis fizyczny: p.89-96,fig.,ref.

Twórcy

autor

Krzyzowska M

• Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Schollenberger A.

autor

Pawlowski A.

autor

Hamasur B.

autor

Winnicka A.

autor

Augustynowicz-Kopec E.

autor

Niemialtowski M.

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