Study of the NRT-PCR method for the detection of the West Nile virus

• Autorzy: Niczyporuk J S , Samorek-Salamonowicz E.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to develop RT-PCR and Nested-PCR methods for the detection and identification of the West Nile virus in wild birds. The method was based on West Nile Virus genome non-coding region 3'NCR. All the samples were examined with NRT-PCR and also with a commercial West Nile Virus Kit Prodesse™. There was no positive case of West Nile virus infection in the examined samples. All control samples were positive in both methods. The NRT-PCR method proved to be quick and reliable for the testing of wild birds' tissue samples for WNV.

Słowa kluczowe

EN

bird; wild animal; West Nile virus; virus; Flaviviridae; identification; detection; method

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Opis fizyczny: p.187-192,fig.,ref.

Twórcy

autor

Niczyporuk J S

• National Veterianry Research Institute, 24-100 Pulawy, Poland

autor

Samorek-Salamonowicz E.

Bibliografia

• 1. Berthet F.X., Zeller H.G., Dronet M.T., Rauzier J., Digoutte J.P., Deubel V.: Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile virus. J Gen Virol 1997, 78, 2293-2297.
• 2. Hayes E.B., O'Leary D.R.: West Nile virus infection: a pediatric perspective. Pediatrics 2004, 113, 1375-1381.
• 3. Hermanowska-Szpakowicz T., Grygorczuk S., Kondrusik M., Zajkowska J., Pancewicz S.: Infections caused by West Nile Virus. Przegl Epidemiol 2006, 60, 93-98.
• 4. Huang C., Slater B., Rudd R., Parchuri N., Hull R., Dupuis M., Hindenburg H.: First isolation of West Nile virus from a patient with encephalitis in the United States. Emerg Infect Dis 2002, 8, 1367-1371.
• 5. Johnson D.J., Ostlund E.N., Pedersen D.D., Schmitt B.J.: Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay. Lancet 2000, 355, 1614-1615.
• 6. Juricova Z., Pinowski J., Literak I., Hahm K.H., Romanowski J.: Antibodies to alphavirus, flavivirus, and bunyavirus arboviruses in house sparrows (Passer domesticus) and tree sparrows (Passer montanus) in Poland. Avian Dis 1998, 42, 182-185.
• 7. Kauffman E.B., Jones S.A., Alan P., Dupuis I.I., Ngo K.E., Bernard K.A., Kramer L.D.: Virus detection protocols for West Nile virus in vertebrate and mosquito specimens. J Clin Microbiol 2003, 41, 3661-3667.
• 8. Lanciotti R.S., Ebel G.D., Deubel V., Kerst A.J., Murri S., Meyer R., Bowen M., McKinney N., Morrill W.E., Crabtree M.B., Kramer L.D., Roehring J.T. Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East. Virology 2002, 298, 96-105.
• 9. Lanciotti R.S., Kest A.J., Nasci R.S., Godsey M.S., Mitchell C.J., Savage H.M., Panella N.A., Allen B.C., Volpe K.E., Davis B.S., Roehring J.T.: Rapid detection of West Nile virus from human clinical samples, field collected mosquitoes and avian samples by Taq Man reverse transcriptase-PCR assay. J Clin Microbiol 2000, 38, 4066-4071.
• 10. Langevin S.A., Bunning M., Davis B., Komar N.: Experimental infection of chickens as candidate sentinels for West Nile virus. Emerg Infect Dis 2001, 7, 726-729.
• 11. Malkinson M., Banet C.: The role of binds in the ecology of West Nile virus in Europe and Africa. Curr Top Microbiol Immunol 2002, 267, 309-322.
• 12. Monath T.P., Arroyo J., Miller C., Guirakhoo F.: West Nile virus vaccine. Curr Drug Targets Infect Disord 2001, 1, 37-50.
• 13. Petersen L.R., Marfin A.A.: West Nile virus: a primer for the clinician. Ann Int Med 2002, 137, 173-179.
• 14. Samorek-Salamonowicz E., Niczyporuk J.S., Wijaszka T.: West Nile Virus - threat for public health. Medycyna Wet 2008, 64, 1368-1370.
• 15. Shi P.Y., Kauffman E.B., Ren P., Felton A., Tai J.H., Dupuis I.I., Jones S.A., Ngo K.A., Nicholas D.C., Maffei J., Ebel G.D., Bernard K.A., Kramer L.D.: High- throughput detection of West Nile virus RNA. J Clin Microbiol 2001, 39, 1264-1271.
• 16. Steele K.E., Linn M.J., Schoepp R.J., Komar N., Geisbert, T.W., Manduca R.M., Calle P.P., Raphael B.L., Clippinger T.L., Larsen T., Smith J., Lanciotti R.S., Panella N.A., McNamara T.S.: Pathology of fatal West Nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York. Vet Pathol 2000, 37, 208-224.
• 17. Walker B.L., Naugle D.E., Doherty K.E., Cornish T.E.: West Nile virus and greater Sage-Grouse: estimating infection rate in a wild bird population. Avian Dis 2007, 51, 691-696.
• 18. Wegner E., Hubalek Z.: X International symposium „Parasitic, allergic and poisonous arthropods - medical and sanitary importance”. Conference materials, Kazimierz Dolny, 2008, p. 51.