The application of the AFLP method to determine the purity of homozygous lines of barley [Hordeum vulgare L.]

• Autorzy: Oleszczuk S , Zimny J. , Bednarek P.T.
• Języki publikacji: EN

Abstrakty

EN

Amplified fragment length polymorphism of DNA has been used to analyse the equality of plants obtained from isolated microspores. Although the control parental material was regarded as being highly homozygous, the analysis of the banding patterns of single plants showed a certain level of polymorphism. The analysis of regenerants with a doubled chromosome number did not show any diversity within the progeny of a single line. The differences in banding patterns coming from single plants were only observed in microspore donor lines. These results have proven the high purity of homozygous lines obtained via androgenesis from isolated microspores.

Słowa kluczowe

EN

genotype stability; plant cytogenetics; homozygous line; isolated microspore; barley; homozygosity; Hordeum vulgare; androgenesis; plant breeding

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.777-783,fig.

Twórcy

autor

Oleszczuk S

• Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland

autor

Zimny J.

autor

Bednarek P.T.

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