IGF-1 stimulates production of interleukin-10 and inhibits development of caerulein-induced pancreatitis

• Autorzy: Warzecha Z. , Dembinski A. , Ceranowicz P. , Konturek S.J. , Tomaszewska R. , Stachura J. , Konturek P.C.
• Języki publikacji: EN

Abstrakty

EN

Insulin-like growth factor-1 (IGF-1) and other growth factors overexpression was reported in acute pancreatitis. Previous studies have shown the protective effect of epidermal growth factor (EGF), Hepatocyte Growth Factor (HGF) and Fibroblast Growth Factor (FGF) in the course of experimental acute pancreatitis. The aim of our studies was to determine the effect of IGF-1 administration on the development of caerulein-induced pancreatitis. Methods: Acute pancreatitis was induced by infusion of caerulein (10 µg/kg/h) for 5 h. IGF-1 was administrated twice at the doses: 2, 10, 50, or 100 µg/kg s.c. Results: Administration of IGF-1 without induction of pancreatitis increased plasma interleukin-10 (IL-10). Infusion of caerulein led to development of acute edematous pancreatitis. Histological examination showed pancreatic edema, leukocyte infiltration and vacuolization of acinar cells. Also, acute pancreatitis led to an increase in plasma lipase and interleukin 1ß (IL-1ß) level, whereas pancreatic DNA synthesis and pancreatic blood flow were decreased. Treatment with IGF-1, during induction of pancreatitis, increased plasma IL-10 and attenuated the pancreatic damage, what was manifested by histological improvement of pancreatic integrity, the partial reversion of the drop in pancreatic DNA synthesis and pancreatic blood flow, and the reduction in pancreatitis-evoked increase in plasma amylase, lipase and IL-1ß level. Protective effect of IGF-1 administration was dose-dependent. Similar strong protective effect was observed after IGF-1 at the dose 2 x 50 and 2 x 100 µg/kg. Conclusions: (1) Administration of IGF-1 attenuates pancreatic damage in caerulein-induced pancreatitis; (2) This effect is related, at least in part, to the increase in IL-10 production, the reduction in liberation of IL-1ß and the improvement of pancreatic blood flow.

Słowa kluczowe

EN

acute pancreatitis; pancreatic blood flow; interleukin 1beta; pancreatitis; interleukin 10; insulin-like growth factor 1; caerulein-induced pancreatitis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2003
• Tom: 54
• Numer: 4
• Opis fizyczny: p.575-590,fig.,ref.

Twórcy

autor

Warzecha Z.

• Jagiellonian University Medical School, 16 Grzegorzecka Street, 31-531 Krakow, Poland

autor

Dembinski A.

autor

Ceranowicz P.

autor

Konturek S.J.

autor

Tomaszewska R.

autor

Stachura J.

autor

Konturek P.C.