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1996 | 43 | 3 |

Tytuł artykułu

The use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Elastase, cathepsin G and azurocidin from human neutrophils are key components of body inflammatory defense. Perturbations in regulation of their activities lead to many serious pathological states. The paper describes a simple, fast and efficient method of joint purification of these proteins with the use of sequential affinity chromatography on squash trypsin inhibitor (CMTI I) and bovine pancreatic trypsin inhibitor (BPTI).

Wydawca

-

Rocznik

Tom

43

Numer

3

Opis fizyczny

p.503-506,fig.

Twórcy

autor
  • Wroclaw University, Tamka 2, 50-137 Wroclaw, Poland
autor

Bibliografia

  • 1. Rest, R. (1988) Human neutrophil and mast cell proteases implicated in inflammation. Methods Lnzymol. 163,309-327.
  • 2. Oderberg, H. & Olsson, I. (1975) Antibacterial activity of cationic proteins from human granulocytes. J. Clin. Invest. 56,1118-1124.
  • 3. Campanelli, D., Detmers, P., Nathan, C.F. & Gabay,J.E.(1990) Azurocidin and a homologous serine protease from neutrophils. }. Clin. Invest. 85,904-915.
  • 4. Pereira, H.A., Shafer, W.M., Pohl,J., Martin, L.E. & Spitznagel, J.K. (1990) CAP37, a human neutrophil-derived chemotactic factor with monocyte specific activity. J. Clin. Invest. 85, 1468-1476.
  • 5. Owen, C.A., Campbell, M.A., Sannes, P.L., Boukedes, S.S. & Campbell, E.J. (1995) Cell surface-bound elastase and cathepsin G on human neutrophils: A novel, non-oxidative mechanism by which neutrophils focus and preserve catalytic activity of serine proteinases. /. Cell Biol. 131,775-789.
  • 6. Klebanoff, S.J. & Clark, R.A. (1978) Neutrophil: Function and Clinical Disorders; pp. 217-280, North-Holland, Amsterdam.
  • 7. Travis, J. & Salvesen, G. (1983) I Iuman plasma proteinase inhibitors. Annu. Rev. Biochem. 52, 655-709.
  • 8. Havemann, K. & Gramse, M. (1984) Physiology and pathophysiology of neutral proteinases of human granulocytes; in Proteases: Potential Role in Health and Disease (Horl, W.H. & Heidland, A., eds.) pp. 1-20, Plenum Press, New York.
  • 9. Baugh, R.J. & Travis, J. (1976) Human leukocyte granule elastase Rapid isolation and characte­rization. Biochemistry 15,836-641. 10. Travis, J., Bowen, J. & Baugh, R. (1978) Human a-l-antichymotrypsin: Interaction with chymo- trypsin-like proteinases. Biochemistry 17, 5651-5656.
  • 11. Polanowski, A., Cie^lar, E., Otlewski, J., Nien- artowicz, B., Wilimowska-Pelc, A. & Wilusz, T. (1987) Protein inhibitors of trypsin from the seeds of Cucurbitaceae plants. Acta Biochim. Po/on. 34,395-406.
  • 12. Wilusz, T., Lomako, J. & Mejbaum-Katzenellen- bogen, W. (1973) An improved method of isolation of crystalline basic trypsin inhibitor from bovine tissues. Acta Biochim. Polon. 20, 25-31.
  • 13. Pepper, D.S. (1992) Some alternative coupling chemistries for affinity chromatography; in Practical Protein Chromatography (Kcriney, A. & Fowcll,S.,eds.) pp.181-183, The Humana Press, Totova, N.J.
  • 14. Gill, S.C. & von llippel, PH. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochent. 182,319-326.
  • 15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227,680-685.
  • 16. Otlewski, J., Zbyryt, T., Krokoszyriska, 1. & Wilusz, T. (1990) Inhibition of serine proteinases by squash inhibitors. Biol. Chem. Hoppc-Seyler 371, 589-594.
  • 17. Petersen, L.C., Birktoft, J.J. & Flodgaard, H. (1993) Binding of bovine pancreatic trypsin inhi- bitor to heparin binding protein/CAP37/ /azurocidin. Interaction between a Kunitz-type inhibitor and a proteolytically inactive serine proteinase homologue. Eur. J. Biochem. 214, 271-279.
  • 18. Pohl, J., Pereira, I I.A., Martin, N.M. & Spitz- nagel, J.K. (1990) Amino acid sequence of CAP37, a human neutrophil granule-derived anti-bacterial and monocyte-specific chemo- tactic glycoprotein structurally similar to neutrophil elastase. FEBS Lett. 172,200-204.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-a37072ad-c1b2-4449-a902-ce4363dbf796
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