Wplyw witamin C i E oraz selenu na aktywnosc leukocytow i status antyoksydacyjny krwi krow w okresie puerperium

• Autorzy: Markiewicz H , Gehrke M. , Malinowski E.

Warianty tytułu

EN

Influence of vitamins C and E and selenium injections before parturition on the activity of leucocytes and antioxidative status of blood in cows in the puerperal period

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to examine the influence of Se, vitamins E and C injections before parturition (in the recommended doses) on the activity of leucocytes and antioxidative status of blood in cows during the puerperal period. Blood samples from 32 cows constituted the material for laboratory tests. Three weeks before the expected parturition 16 cows were treated with a single injection of 500 mg of vitamin E as tocopherol acetate, and 5 mg of sodium selenite. In addition, the animals were injected intramuscularly with 2.5 g of vitamin C for five consecutive days. The control group consisted of 16 cows that did not receive the above mentioned antioxidants. Blood samples were collected twice before the parturition and in 1, 2 and 4 weeks after it. T lymphocytes subpopulations were evaluated by flow cytometry (FACS Calibur Becton Dickinson). Total antioxidative status was determined on the basis of TAS, SOD, GSH-Px and CAT activity (using Randox and Oxis test kits). Spontaneous and induced chemiluminescence (CL) of neutrophils in whole blood was measured (Luminometr BioOrbit 1251) using opsonized zymosan (OZ) and phorbol myristate acetate (PMA) as stimulators. The study showed that the primary manner to induce phagocytic activity during the puerperal period is through an extrareceptor mechanism. After parturition the antioxidative defense system is weakened and injections of antioxidative preparations improve the pro-antioxidative balance of the organism. The injected Antioxidants also increased T lymphocytes proliferation. The antioxidative preparations in the applied doses do not stimulate the oxidative burst of the granulocytes. The obtained results suggest that the injection of vitamins C, E and Se in doses recommended by manufacturers should be made in the last week before parturition.

Słowa kluczowe

PL

leukocyty; witamina E; okres okoloporodowy; bydlo; stan antyoksydacyjny; selen; witamina C; badania krwi; krowy

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 05
• Opis fizyczny: s.566-570,tab.,bibliogr.

Twórcy

autor

Markiewicz H

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy w Pulawach, Al.Powstancow Wlkp. 10, 85-090 Bydgoszcz

autor

Gehrke M.

autor

Malinowski E.

Bibliografia

• 1.Allison R. D., Laven R. A.: Effect of vitamin E supplementation on the health and fertility of dairy cows: a review. Vet. Rec. 2000, 147, 703-708.
• 2.Bernabucci U., Ronchi B., Lacetera N., Nardone A.: Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J. Dairy Sci. 2005, 88, 2017-2026.
• 3.Bernabucci U., Ronchi B., Lacetera N., Nardone A.: Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. J. Dairy Sci. 2002, 85, 2173-2179.
• 4.Brzezinska-Slebodzinska E., Miller J. K., Quigley J. D., Moore J. R.: Antioxidant status of dairy cows supplemented prepartum with vitamin E and selenium. J. Dairy Sci. 1994, 77, 3087-3095.
• 5.Bostedt H., Max A., Gajewski Z.: Wpływ żywienia na płodność krów mlecznych. Nowa Weterynaria 1997, III Polsko-Niemieckie Sympozjum Fizjologia i Patologia Rozrodu Zwierząt ''Problemy rozrodu bydła'', s. 14-18.
• 6.Castillo C., Hernandez J., Bravo A., Lopez-Alonso M., Pereira V., Benedito J. L.: Oxidative status during late pregnancy and early lactation in dairy cows. Veterinary J. 2005, 169, 286-292.
• 7.Goff J. P., Kimura K., Horst R. L.: Effect of mastectomy on milk fever, energy, and vitamins A, E, and b-carotene status at parturition. J. Dairy Sci. 2002, 85, 1427-1436.
• 8.Hajurka J., Macák V., Hura V.: Influence of health status of reproductive organs on uterine involution in airy cows. Bull. Vet. Inst. Pulawy 2005, 49, 53-58.
• 9.Hajurka J., Macák V., Hura V.: Uterine and ovarian factors influencing involution of the uterus in cows. Achievements and Prospects of Ruminants Medicine, Monograph, Puławy 2005, 325-327.
• 10.Hogan J. S., Weiss W. P., Todhunter D. A., Smith K. L., Schoenberger P. S.: Bovine neutrophil responses to parenteral vitamin E. J. Dairy Sci. 1992, 75, 399-405.
• 11. Jukola E., Hakkarainen J., Saloniemi H., Sankami S.: Blood selenium, witamin E, witamin A, and b-carotene concentrations and udder health, fertility treatments, and fertility. J. Dairy Sci. 1996, 79, 838-845.
• 12.Kimura K., Goff J. P., Kehrli M. E., Harp J. A.: Phenotype analysis of peripheral blood mononuclear cells in periparturient dairy cows. J. Dairy Sci. 1999, 82, 315-319.
• 13.Kimura K., Goff J. P., Kehrli M. E., Harp J. A., Nonnecke B. J.: Effects of mastectomy on composition of peripheral blood mononuclear cell populations in periparturient dairy cows. J. Dairy Sci. 2002, 85, 1437-1444.
• 14.Kleczkowski M., Kluciñski W., Sikora J., Zdanowicz M.: Role of antioxidants in the protection against oxidative stress in cattle trace elements and enzymatic mechanisms. Polish J. Vet. Sci. 2004, 7, 233-240.
• 15.Krakowski L., Wrona Z., Kostro K., Krakowska I., Kostrzewa A., Piech T., Brodzki P.: Ocena subpopulacji limfocytów TCD4+ i TCD8+ oraz aktywności fagocytarnej neutrofilów i monocytów w krwi obwodowej krów i jałówek w okresie okołoporodowym. Medycyna Wet. 2004, 60, 415-419.
• 16.Lacetera N., Scalia D., Bernabucci U., Ronchi B., Pirazzi D., Nardone A.: Lymphocyte functions in overconditioned cows around parturition. J. Dairy Sci. 2005, 88, 2010-2016.
• 17.LeBlanc S. J., Herdt T. H., Seymour W. M., Duffield T. F., Leslie K. E.: Peripartum serum vitamin E, retinol, and beta-carotene in dairy cattle and their associations with disease. J. Dairy Sci. 2004, 87, 609-619.
• 18.Liu L., Scheffer K. K., Shuefea D. M.: Jugular infusion of vitamin C and color stability of beef. J. Anim. Sci. 1994, 72, 372 (abstr.).
• 19.Luck M. R., Jeyaseelan I., Scholes R. A.: Ascorbic acid and fertility. Biol. Reprod. 1995, 52, 262-266.
• 20.Markiewicz H., Gehrke M., Malinowski E., Kaczmarowski M.: Ocena potencjału antyoksydacyjnego krwi krów w okresie okołoporodowym. Medycyna Wet. 2005, 61, 1382-1384.
• 21.Mehrzad J., Duchateau L., Pyörälä S., Burvenich C.: Blood and milk neutrophil chemiluminescencje and viability in primiparous and pluriparous dairy cows during late pregnancy, around parturition and early lactation. J. Dairy Sci. 2002, 85, 3268-3276.
• 22.Miller J. K., Brzezinska-Slebodzinska E., Madsen F. C.: Oxidative stress, antioxidants, and animal function. J. Dairy Sci. 1993, 76, 2812-2823.
• 23.Muehlenbein E. L., Brink D. R., Deutscher G. H., Carlson M. P., Johnson A. B.: Effects of inorganic and organic copper supplemented to first-calf cows on cow reproduction and calf health and performance. J. Anim. Sci. 2001, 79, 1650- -1659.
• 24.Nonnecke B. J., Kimura K., Goff J. P., Kehrli M. E.: Effects of the mammary gland on functional capacities of blood mononuclear leukocyte populations from periparturient cows. J. Dairy Sci. 2003, 86, 2359-2368.
• 25.Park Y. H., Fox L. K., Hamilton M. J., Davis W. C.: Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation. J. Dairy Sci. 1992, 75, 998-1006.
• 26.Paula-Lopes F. F., Al-Katanani Y. M., Majewski A. C., McDowell L. R., Hansen P. J.: Manipulation of antioxidant status fails to improve fertility of lactating cows or survival of heat-shocked embryos. J. Dairy Sci. 2003, 86, 2343- -2351.
• 27.Van Kampen C., Mallard B. A.: Effects of peripartum stress and health on circulating bovine lymphocyte subsets. Vet. Immunol. Immunopathol. 1997, 59, 79-91.
• 28.Washko P. W., Wang Y., Levine M.: Ascorbic acid recycling in human neutrophils. J. Biol. Chem. 1993, 268, 15531-15535.
• 29.Weiss W. P.: Effect of dietary witamin C on concentrations of ascorbic acid in plasma and milk. J. Dairy Sci. 2001, 84, 2302-2307.
• 30.Weiss W. P., Hogan J. S., Smith K. L.: Changes in vitamin C concentrations in plasma and milk from dairy cows after an intramammary infusion of Escherichia coli. J. Dairy Sci. 2004, 87, 32-37.