Effects of arginine substitutions on the cardioinhibitory activity of the Led-NPF-I neuropeptide

• Autorzy: Marciniak P. , Slocinska M. , Bednarz P. , Grodecki S. , Konopinska D. , Rosinski G.
• Języki publikacji: EN

Abstrakty

EN

Effects of structural changes on the cardioinhibitory activity of the Led-NPF-I peptide (Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-amide) were examined by replacing Arg residues in positions 2, 7 and 9. Replacement of L-Arg2 with another basic amino acid, such as Lys, His or D-Arg, did not abolish but rather promoted cardioinhibitory activity in giant mealworm beetle Zophobas atratus Fab. Agonistic peptides were also obtained by substitution of Arg residue in position 7 with Lys or D-Arg, and Arg in position 9 with His or D-Arg, respectively. All these analogues showed stronger cardioinhibitory effects than the native peptide at low concentration (10-9 M), and [Lys7]-, [D-Arg7]- and [D-Arg9]-Led-NPF-I also at the higher concentration (10-6 M). However, substitutions of the Arg residues in position 7 with His or in position 9 with Lys caused a loss of the cardioinhibitory activity. In addition, the replacement of Arg residues in all three positions with Lys or Orn caused a reduction of cardioinhibitory activity, although a single substitution of Arg in positions 2 or 7 with Lys yielded agonistic peptides. We conclude that the Arg2 position in the N-terminal region is more tolerant to structural modification than the other two Arg positions located in the C-terminal region.

Słowa kluczowe

EN

cardioinhibitory activity; arginine substitution; mealworm beetle; Zophobas atratus; giant mealworm beetle; Led-NPF-I-peptide; insect; beetle; neuropeptide

• Wydawca: -
• Czasopismo: Pestycydy
• Rocznik: 2009
• Numer: 1-4
• Opis fizyczny: p.71-78,fig.,ref.

Twórcy

autor

Marciniak P.

• Adam Mickiewicz University, 89 Umultowska St., 61-614 Poznan, Poland

autor

Slocinska M.

autor

Bednarz P.

autor

Grodecki S.

autor

Konopinska D.

autor

Rosinski G.

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