Ocena skutecznosci szczepionki przeciwko CyHV-3 u narybku karpia [Cyprinus carpio L.] - badania doswiadczalne

• Autorzy: Siwicki A K , Kotler Y. , Terech-Majewska E. , Kazun K. , Glabski E. , Kazun B.

Warianty tytułu

EN

Evaluation of the efficacy of a vaccine against CyHV-3 in carp fingerlings [Cyprinus carpio L.] - an experimental study

• Języki publikacji: PL

Abstrakty

EN

The study focuses on how to improve the potency and efficacy of a vaccine against CyHV-3 in carp fingerlings. The study material comprised the KoVax vaccine (Israel) applied through immersion (1 ml of vaccine diluted in 10 l of water) and 1 kg of carp fingerlings with individual weights of about 10 g. The immersion time was 40 minutes at different temperatures (16°C, 18°C, 20°C, 22°C). After 21 days, 50 fish from each temperature variant were infected experimentally through injections of CyHV-3 isolated from Polish carp at the same temperatures, and 50 fish were infected likewise from each group and held at a temperature of 22°C. The control group comprised fish that had only been vaccinated. The fish were held in tanks with a volume of 500 l (100 fish per tank) and were fed daily with commercial pellets. Mortality was tabulated and monitored, and the presence of the pathogen was confirmed through isolation from the gills and pronephros. The results of the study indicated that the temperature at vaccination is very important for achieving protection from CyHV-3. The highest mortality was observed in fish vaccinated at 16°C and then infected with live virus at 22°C (80%) in comparison to the group of fish vaccinated at 18°C (68%) or 20°C (40%). The fish vaccinated and infected at similar temperatures presented different levels of protection against CyHV-3. Mortality was the lowest at 22°C (20%), in comparison to that at 16°C (32%), 18°C (40%), or 20°C (38%).

Słowa kluczowe

PL

ocena skutecznosci; wirus CyHV-3; narybek; Cyprinus carpio; karp; badania doswiadczalne; szczepionki przeciwwirusowe

• Wydawca: -
• Czasopismo: Komunikaty Rybackie
• Rocznik: 2009
• Numer: 5
• Opis fizyczny: s.2-4,rys.,tab.,bibliogr.

Twórcy

autor

Siwicki A K

• Instytut Rybactwa Srodladowego, Olsztyn

autor

Kotler Y.

autor

Terech-Majewska E.

autor

Kazun K.

autor

Glabski E.

autor

Kazun B.

Bibliografia

• Antychowicz J., Reichert M., Matras M., Bergmann S.M., Haenen O. 2005 - Epidemiology, pathogenecity and molecular biology of koi herpesvirus isolated in Poland - Bull. Vet. Inst. Puławy 49: 367-373.
• Bergmann S.M., Kempter J., Sadowski J., Fichtner J. 2006 - First detection, confirmation and isolation of koi herpesvirus (KHV) in cultured common carp (Cyprinus carpio) in Poland - Bull. Eur. Ass. Fish Pathol. 26:97-104.
• Gudding R., Lillehaug A., Evensen O. 1999 - Recent developments in fish vaccinology - Vet. Immunol. Immunopathol. 72: 203-2012.
• Pikarsky E., Ronen A., Abramowitz J., Softer D., Kotler M. 2004 - Pathogenesis of acute viral disease induced in fish by carp intestinal nephritis and gill necrosis virus - J. Virol. 78: 9544-9551.
• Ronen A., Perelberg A., Abramowitz J., Hutoran M., Tinman S., Bejerano I., Stelnitz M., Kotler M. 2003 - Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio - Vaccine 21: 4677-4684.
• Siwicki A.K., Lepa A., Małaczewska J., Kazuń B., Kazuń K., Terech-Majewska E. 2006 - Isolation and Identification of carp interstitial nephritis and gill necrosis virus (CNGV) in fingerling common carp (Cyprinus carpio L.) - Arch. Pol. Fish. 14: 157-167.
• Siwicki A.K., Małaczewska J., Lepa A. 2008a - Influence of CyHV-3 on macrophage and lymphocyte activity In carp (Cyprinus carpio): an in vitro study - Cyprinid herpes viruses: basic and applied aspect, International Workshop on CyHV-3 (KHV), Hebrew University of Jerusalem 2008:31.
• Siwicki A.K., Małaczewska J., Kazuń B., Wójcik R. 2008b - Pathogenesis of CyHV-3: In vitro effect on phagocyte activity and cytokine-like protein production in carp (Cyprinus carpio), tench (Tinca tinca) and sheat- fish (Silurus glanis) - Cyprinid herpes viruses: basic and applied aspect, International Workshop on CyHV-3 (KHV), Hebrew University of Jerusalem 2008: 34.