Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus

• Autorzy: Gromadzka B. , Szewczyk B. , Konopa G. , Fitzner A. , Kesy A.
• Języki publikacji: EN

Abstrakty

EN

Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.

Słowa kluczowe

EN

Lagovirus; Caliciviridae; rabbit hemorrhagic disease virus; domestic rabbit; virus-like particle; baculovirus; wild rabbit; rabbit hemorrhagic disease; animal disease; rabbit

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2006
• Tom: 53
• Numer: 2
• Opis fizyczny: p.371-376,fig.,ref.

Twórcy

autor

Gromadzka B.

• Medical University of Gdansk, Gdansk, Poland

autor

Szewczyk B.

autor

Konopa G.

autor

Fitzner A.

autor

Kesy A.

Bibliografia

• Capucci L, Scicluna MT, Lavazza A (1991) Diagnosis of viral haemorrhagic disease of rabbits and the European brown hare syndrome. Rev Sci Tech 10: 347–370.
• Capucci L, Frigoli G, Ronshold L, Lavazza A, Brocchi E, Rossi C (1995) Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies. Virus Res 37: 221–238.
• Capucci L, Fallacara F, Grazioli S, Lavazza A, Pacciarini ML, Brocchi E (1998) A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant. Virus Res 58: 115–126.
• Castanon S, Marin MS, Martin-Alonso JM, Boga A, Casais R, Humara JM, Ordas RJ, Parra F (1999) Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus. J Virol 73: 4452–4455.
• Fitzner A, Kęsy A, Niedbalski W, Paprocka G (1999) Zastosowanie reakcji RT-PCR do identyfikacji szczepów wirusa krwotocznej choroby królików (RHD) wyosobnionych w Polsce. Medycyna Wet 55: 120–122 (in Polish).
• Jiang X, Graham DY, Wang M, Estes MK (1992) Expression, self assembly and antigenicity of the Norwalk virus capsid protein. J Virol 66: 6527–6532.
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
• Laurent S, Vautherot J-F, Madelaine M-F, Le Gall G, Rasschaert D (1994) Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protecion. J Virol 68: 6794–6798.
• Laurent S, Vautherot J-F, Gall le G, Madelaine M-F, Rasschaert D (1997) Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus. J Gen Virol 78: 2803–2811.
• Le Gall G, Boilletot E, Morisse J-P (1992) Viral haemorrhagic disease of rabbit: purification and characterization of a strain isolated in France. Ann Rech Vet 23: 381–387.
• Liu SJ, Xue HP, Pu BQ, Quian NH (1984) A new viral disease in rabbits. Anim Husb Vet Med 16: 253–255.
• Martinez-Torrecuadrada JL, Cortes E, Vela C, Langeveld JPM, Meloen RH, Dalsgaard K, Hamilton WDO, Casal JI (1998) Antigenic structure of the capsid protein of rabbit haemorrhagic disease virus. J Gen Virol 79: 1901–1909.
• Meyers GC, Wirblich H, Thiel H-J (1991a) Rabbit hemorrhagic disease virus molecular-cloning and nucleotide sequencing of a calicivirus genome. Virology 184: 664–676.
• Meyers G, Wirblich CH, Thiel H-J (1991b) Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology 184: 677–686.
• Nagesha HS, Wang LF, Hyatt AD, Morrissy CJ, Lenghaus C, Westbury HA (1995) Self assembly, antigenicity, and immunogenicity of the rabbit haemorrhagic disease virus (Czechoslovakian strain V-351) capsid protein expressed in baculovirus. Arch Virol 140: 1095–1108.
• Ohlinger VF, Haas B, Meyers G, Weiland F, Thiel H-J (1990) Identification of the virus causing rabbit hemorrhagic disease. J Virol 64: 3331–3336.
• Parra F, Prieto M (1990) Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. J Virol 64: 4013–4015.
• Sibilia M, Boniotti MB, Angoscini P, Capucci L, Rossi C (1995) Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein. J Virol 69: 5812–5815.