Opornosc paleczek Enterobacteriaceae wyosobnionych w szpitalu polozniczo-ginekologicznym na wybrane antybiotyki aminoglikozydowe ze szczegolnym uwzglednieniem zdolnosci do wytwarzania 16S rRNA metylaz ArmA, RmtB i RmtC

• Autorzy: Zacharczuk K. , Piekarska K. , Szych J. , Sulik M. , Roszko E. , Lata J. , Jagielski M. , Gierczynski R.

Warianty tytułu

EN

Aminoglycoside resistance in Enterobacteriaceae originated in a gynecology-obstetrical hospital: a survey for 16S rRNA methylases: ArmA, RmtB and RmtC

• Języki publikacji: PL

Abstrakty

PL

Określono udział szczepów opornych na wybrane aminoglikozydy (gentami- cyna, amikacyna, netylmycyna, tobramycyna i neomycyna) u 2359 izolatów pałeczek Enterobacteriaceae wyosobnionych z materiału klinicznego od pacjentek szpitala ginekologiczno-położniczego i od noworodków. Oporność na jeden lub więcej z wymienionych antybiotyków stwierdzono u 45 izolatów (1,90%), z czego 33 (73,33%) należało do gatunku Escherichia coli. W metodzie dyfuzyjne—krążkowej 14 izolatów (31,11%) wykazywało wzrost do krawędzi krążka z gentamicyną (10 µg), w tym 7 izolatów charakteryzowało się wartością MIC w zakresie od 256 do 1024 µg/ml, jednakże u tych izolatów nie stwierdzono obecności 16S rRNAmetylaz Arm A, RmtB lub RrmtC. Uzyskane wyniki wskazują na nieznaczny udział szczepów opornych na aminoglikozydy wśród pałeczek Enterobacteriaceae występujących u kobiet w okresie okołoporodowym i mogą świadczyć o znikomym rozprzestrzenieniu pałeczek wytwarzających 16S rRNA metylazy w populacji.

EN

We examined the resistance of 2359 clinical isolates of Enterobacteriaceae to gentamicin, amikacin, netilmicin and neomycin by the disc-diffusion assay. The isolates originated from female-patients and newborn infants in a gynecology-obstetrical hospital in Warsaw, Poland. Isolates from adults predominated. Most of the isolated bacteria were considered non-pathogenic, colonization flora. The majority (above 95%) of the isolates were sensitive to all of the tested aminoglycosides. Forty five (1,90%) isolates were resistant to one or more agents. In this group, E. coli was prevalent (73,33%). As little as 14 isolates had no growth inhibition around the gentamicin disc (10 mcg) (contact-growth). MICs of seven isolates ranged from 256 to 1024 (mcg/ml) of the tested agents. One isolate had MIC 1024 for amikacin and kanamycin. All the contact-growth isolates were examined for genes encoding for TEM, SH V and CTX-M beta-lactamases, and genes armA, rmtB and rmtC for 16S rRN A methy lases reported in Europe. All of them produced ТЕМ enzyme while SHV and CTX-M was expressed by one and two isolates respectively. None of the tested 16S rRNA methylases was detected. Our results show the low carriage of the aminoglycoside-resistant Enterobacteriaceae in healthy pregnant-women and most probably their sexual partners. Our findings may argue that production of the 16S rRNA methylases is still limited to patients with a long-term antibiotic-therapy in regular hospitals rather than to non-hospitalized population in Poland.

Słowa kluczowe

PL

Enterobacteriaceae; lekoopornosc; szpitale polozniczo-ginekologiczne; antybiotyki aminoglikozydowe; opornosc na antybiotyki; szczepy bakteryjne; izolaty bakteryjne; pacjenci; Escherichia coli; 16S rRNA; okres okoloporodowy

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2010
• Tom: 62
• Numer: 2
• Opis fizyczny: s.97-107,rys.,tab.,bibliogr.

Twórcy

autor

Zacharczuk K.

• Zaklad Bakteriologii, Narodowy Instytut Zdrowia Publicznego - Panstwowy Zaklad Higieny, ul.Chocimska 24, 00-791 Warszawa

autor

Piekarska K.

autor

Szych J.

autor

Sulik M.

autor

Roszko E.

autor

Lata J.

autor

Jagielski M.

autor

Gierczynski R.

Bibliografia

• 1. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank. Nucleic Acids Res 2008; 36:D25-30
• 2. Bogaerts P., Galimand M., Bauraing C i inni. Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium. JAC 2007; 59:459-64.
• 3. CLSI. Performance Standards for Antimicriobial Disc Suseptibility Tests, Eighteenth Informational Supplement, CLSI document M100-S18. Clinical and Laboratory Standards Institute Wayne, PA, USA, 2008
• 4. Doi Y, de Oliveira Garcia D, Adams J, Paterson DL. Coproduction of novel 16S rRNA methylase RmtD and metallo-beta-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil. Antimicrob Agents Chemother 2007; 51:852-6
• 5. Doi Y, Yokoyama K., Yamane K., Wachino J., Shibata N., Yagi T., Shibayama K., Kato H., Arakowa Y. Plasmid mediated 16S rRNA methylase in Seratia marcescens confering high level resistance to aminoglycosides. Antimicrob Agents Chemother 2004; 48:491-6
• 6. Doublet B., Weill F.-X., Fabre L., Chaslus-Dancla E., Cloeckaert A. Variant Salmonella genomic island 1 antibiotic resistance gene cluster containing a novel З'-N-aminoglycoside acetyltransferase gene cassette, aac(3)-Id, in Salmonella enterica serovar Newport. Antimicrob Agents Chemother 2004; 48:3806-12
• 7. Du XD, Wu CM, Liu HB, Li XS, Beier RC, Xiao F, Qin SS, Huang SY, Shen JZ. Plasmid-mediated ArmA and RmtB 16S rRNA methylases in Escherichia coli isolated from chickens. J Antimicrob Chemother 2009; 64:1328-30
• 8. Francois В, Russell RJM., Murray JB, Aboulela F, Masquida B, Vicens Q, Westhof E. Crystal structures of complexes between aminoglycosides and decoding A site oligonucleotides: role of the number of rings and positive charges in the specific binding leading to miscoding. Nuc Acids Res 2005; 17:5677-90
• 9. Fritsche TR, Castanheira M, Miller GH, Jones RN, Armstrong ES. Detection of methyltransferases conferring high-level resistance to aminoglycosides in Enterobacteriaceae from Europe, North America, and Latin America. Antimicrob Agents Chemother 2008; 52:1843-5
• 10. Galimand M, Corvalin P, Lambert T. Plasmid-mediated high level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob Agents Chemother 2003; 47:2565-71
• 11. Galimand M, Sabtcheva S, Courvalin P, Lambert T. Wordlwide Disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn 1548. Antimicrob Agents Chemother 2005; 49:2949-53
• 12. Gierczyński R, Szych J, Rastawicki W, Jagielski M. The molecular characterisation of the extended spectrum belactamase (ESBL) producing strain of Salmonella enterica serovar Mbandaka isolated in Poland. Acta Microbiol Polonica 2003; 52:183-90
• 13. Gierczyński R, Szych J, Wardak S, Ławrynowicz-Paciorek M. Occurrence of 16S rRNA methylase ArmA in human clinical isolates of Enterobacteriaceae in Poland. Med.Vet.Net. Third Annual Scientific Meeting, 27-30 czerwiec 2007 , Lucca, Włochy, MVN- Abstract-Book str. 23
• 14. Gołębiewski M, Kern-Zdanowicz I, Zienkiewicz M, Adamczyk M, Żylińska J, Baraniak A, Gniadkowski M, Bardowski J, Cegłowski P. Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum ß-lactamase gene blarCTX-M-3. Antimicrob Agents Chemother 2007; 51:3789-95
• 15. Gonzalez-Zorn B, Tes hager T, Cascas M, Porrero MC, Moreno MA, Courvalin P, Dominguez L. armA and aminoglycoside resistance in Escherichia coli. Emerg Infect Dis 2005; 11:954-56
• 16. Hidalgo L, Gieczyński R, Gutierrez В, Escudero JA, San MIlan A, Szych J, Wardak S, Gonzalez-Zorn В. Characterization of a novel aac(3)-IId gene encoding a high-level gentamicin resistance modifying enzyme in a E coli clinical isolate from Poland. 4th Med-Vet-Net Annual Scientific Conference, 11-14 czerwiec 2008, Saint Malo, Francja MVN-Abstract-Book str. 50.
• 17. Kowalska-Krochmal В, Dolna I, Dobosz A, Wrzyszcz E, GoŚciniak G. Ocena stopnia wrażliwości na aminoglikozydy szczepów bakteryjnych izolowanych od chorych z zakażeniami układowymi i uogólnionymi. Med Dośw Mikrobiol 2008; 60:205-14
• 18. Ma L, Lin CJ, Chen JH i inni. Widespread dissemination of aminoglycoside resistance genes armA and rmtB in Klebsiella pneumoniae isolates in Taiwan producing CTX-M-type extended- spectrum ß-lactamases. Antimicrob Agents Chemother 2009; 53:104-11
• 19. Meszaros J, Hryniewicz W. Antybiotyki w profilaktyce i leczeniu zakażeń. PZWL Warszawa 2001; str. 216-249
• 20. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. Aminoglycosides: activity and resistance. Antmicrob Agents Chemother 1999; 43:727-37
• 21. Piekarska K, Zacharczuk K, Szych J, Zawidzka E, Wilk E, Wardak S, Jagielski M, Gierczyński R. Występowanie w jednym z warszawskich szpitali pałeczek Klebsiella pneumoniae wytwarzających karbapenemazę typu KPC. Med Dośw Mikrobiol 2010; 62:9-20
• 22. Poole K. Aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 2005; 49:479-87
• 23. Sabtcheva S, Saga T, Kantardjiev T, Ivanova M, Ishii Y Kaku M. Nosocomial spread of armA- mediated high-level aminoglycoside resistance in Enterobacteriaceae isolates producing CTX-M-3 ß-lactamase in a cancer hospital in Bulgaria. J Chemother. 2008; 20:593-9
• 24. Shaw K.J, Wright GD. Aminoglycoside resistance in Gram-positive bacteria. W: „Gram-positive pathogens. Red. Fischetti VA., Novick R.P., Ferretti J. J., Portnoy D.A., Rood J.I. ASM Press, Washington D.C. 2000 str. 635-646
• 25. Wachino J, Shibayama K, Kurokawa H, Kimura K, Yamane K, Suzuki S, Shibata N, Ike Y, Arakawa Y. Novel plasmid-mediated 16S rRNA ml Al 408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides. Antimicrob Agents Chemother 2007; 51:4401 -9
• 26. Wachino J., Yamane K, Shibayama K, Kurokawa H., Shibata N., Suzuki S., Doi Y, Kimura K., Ike Y., Arakawa Y. Novel plasmid-mediated 16S rRNA methylase , RmtC, found in a Proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides. Antimicrob Agents Chemother 2006; 50:178-84
• 27. Yamane K, Doi Y, Yokoyama K, Yagi T, Kurokawa H, Shibata N, Shibayama K, Kato H, Arakawa Y Genetic environments of the rmtA gene in Pseudomonas aeruginosa clinical isolates. Antimicrob Agents Chemother 2004; 48:2069-74
• 28. Yan JJ, Wu JJ, Ko WCh, Tsai SH, Chuang ChL, Wu HM, Lu YJ, Li JD. Plasmid mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 2004; 54:1007-12
• 29. Yu F, Wang L, Pan J i inni. Prevalence of 16S rRNA methylase genes in Klebsiella pneumoniae isolates from a Chinese teaching hospital: coexistence of rmtB and armA genes in the same isolate. Diagn Microbiol Infect Dis. 2009; 64:57-63
• 30. Yamane K, Wachino J, Suzuki S, Shibata N, Kato H, Shibayama K, Kimura K Kai K, Ishikawa S, Ozawa Y Konda T, Arakawa Y. 16S rRNA methylase-producing, gram-negative pathogens, Japan. Emerg Infect Dis 2007; 13:642-6