Postnatal expression pattern of the C-EBP alpha gene in porcine subcutaneous adipose tissue

• Autorzy: Jiang J. F. , Xu Z.R. , Wang Y.Z. , Han X.Y. , Wang L.
• Języki publikacji: EN

Abstrakty

EN

CCAAT/enhancer binding protein α (C/EBPα) has been implicated as a key regulator of adipocyte differentiation and lipid metabolism. The pattern of C/EBPα gene expression in different growth stages and its relation to adipose deposition was studied. Fifteen female Duroc × Landrace × Yorkshire pigs in five groups of three pigs each, weighing 1, 30, 50, 70 and 90 kg were used to study developmental gene expression of C/EBPα in subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed that the C/EBPα mRNA levels in porcine adipose tissue continuously increased as the pigs grew and deposited fat from 1 to 90 kg body weight (BW) (P<0.05). The presented data show a close positive correlation between the levels of C/EBPα gene expression and the fat deposition rate in pigs. The experiment showed that total adipose weight increased significantly with BW (P<0.05).

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2006
• Tom: 15
• Numer: 1
• Opis fizyczny: p.61-70,fig.,ref.

Twórcy

autor

Jiang J. F.

• Zhejiang University, Qiutao North Road 164, Hangzhou, 310029, China

autor

Xu Z.R.

autor

Wang Y.Z.

autor

Han X.Y.

autor

Wang L.

Bibliografia

• Antonson P., Xanthopoulos K.G., 1995. Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem. Biophys. Res. Commun. 215, 106-113
• Ding S.-T., McNeel R.L., Mersmann H.J., 1999. Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo. Comp. Biochem. Physiol. Pt B 123, 307-318
• Khoo B.Y., Samian M.R., Najimudin N., Tengku Muhammad T.S., 2003. Molecular cloning and characterisation of peroxisome proliferator activated receptor gamma1 (PPARγ1) cDNA gene from guinea pig (Cavia porcellus): determination of tissue distribution of PPARγ in guinea pig. Comp. Biochem. Physiol. Pt B 134, 37-44
• Kim J.B., Spiegelman B.M., 1996. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Gene. Develop. 10, 1096-107
• Lee K., Hausman G.J., Dean R.G., 1998. Expression of C/EBPs in fetal and postnatal subcutaneous adipose tissue. Mol. Cell. Biochem. 178, 269-74
• Lin F.T., Lane M.D., 1992. Antisense CCAATrenhancer binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 adipocytes. Gene. Develop. 6, 533-544
• MacDougald O.A., Lane M.D., 1995. Transcriptional regulation of gene expression during adipocyte differentiation. Annu. Rev.Biochem. 64, 345-373
• Matsusue K., Gavrilova O., Lambert G., Brewer H.B., Ward J.M., Inoue Y., Leroith D., Gonzalez F.J., 2004. Hepatic CCAAT/enhancer binding protein alpha mediates induction of lipogenesis and regulation of glucose homeostasis in leptin-deficient mice. Mol. Endocrinol. 18, 2751-2764
• SAS Institute Inc., 1989. SAS User’s Guide, Version 6. 4th Edition. Vol 2. Cary, NC Souza D.N., Pethick D.W., Dunshea F.R., Suster D., Pluske J.R., Mullan B.P., 2004. The pattern of fat and lean muscle tissue deposition differs in the different pork primal cuts of female pigs during the finisher growth phase. Livest. Prod. Sci. 91, 1-8
• Tengku Muhammad T.S., Hughes T.R., Ranki H., Cryer A., Ramji D.P., 2000. Differential regulation of macrophage CCAAT-Enhancer binding protein isoforms by lipopolysaccharide and cytokines. Cytokine 12, 1430-1436
• Tontonoz P., Hu E., Spiegelman B.M., 1994. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell 79, 1147-1156
• Umek R.M., Friedman A.D., McKnight S.L., 1991. CCAAT-enhancer binding protein: a component of a differentiation switch. Science 251, 288-292
• Vinson C.R., Sigler P.B., McKnight S.L., 1989. Scissors-grip model for DNA recognition by a family of leucine zipper proteins. Science 246, 911-916
• Wang B.L., Shao J.B., 1989. The research on the growth developmental law in the commercial lean pigs. Ning xia Agr. Ind. Technol. 10, 34-38
• Wang N., Finegold M.J., Bradley A. Ou. C., Abdelsayed S.V., Wilde M.D., Taylor L.R., Wilson D., Darlington G.J., 1995. Impaired energy homeostasis in C/EBP alpha knockout mice. Science 269, 1108-1112
• Yu Z.K., Hausman G.J., 1998, Expression of CCAAT/enhancer binding proteins during porcine preadipocyte differentiation. Exp. Cell Res. 245, 343-349
• Zhang F.J., Pan T.L., Nielsen L.D., Mason R.J., 2004. Lipogenesis in fetal rat lung - Importance of C/EBP alpha SREBP-1c, and stearoyl-CoA desaturase. Amer. J. Respir. Cell Molec. Biol. 30, 174-183