Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis

• Autorzy: Hiszczynska-Sawicka E , Kur J. , Pietkiewicz H. , Holec L. , Gasior A. , Myjak P.
• Języki publikacji: EN

Abstrakty

EN

The description of very efficient system for production and purification of Toxoplasma gondii recombinant antigens, GRA6, p35 and SAG2 is given in this study. The usefulness of these antigens for diagnostic of human infections was tested in an ELISA using 99 sera obtained during routine diagnostics. The sera for testing were selected from either acute or chronically infected patients. Both r-GRA6 and r-p35 antigens detected antibodies more frequently (p＜0.01) from acute (93.9 and 87.9%) rather than chronic (60.6 and 53.0%) infections. The r-SAG2 gave a similar sensitivity in both groups of patients (93.9 and 95.5%).

Słowa kluczowe

EN

toxoplasmosis; antigen; serological detection; serodiagnosis; application; Toxoplasma gondii; recombinant antigen; ELISA test; fusion protein

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2005
• Tom: 50
• Numer: 3
• Opis fizyczny: p.249-254,fig.,ref.

Twórcy

autor

Hiszczynska-Sawicka E

• Technical University of Gdansk, 11/12 Narutowicza Street, 80-952 Gdansk, Poland

autor

Kur J.

autor

Pietkiewicz H.

autor

Holec L.

autor

Gasior A.

autor

Myjak P.

Bibliografia

• Aubert D., Maine G.T., Villena I., Hunt J.C., Howard L., Sheu M., Brojanac S., Chovan L.E., Nowlan S.F., Pinon J.M. 2000. Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. Journal of Clinical Microbiology, 38, 1144-1150.
• Dąbrowski S., Kur J. 1999. Cloning, overexpression, and purification of the recombinant His-tagged SSB protein of Escherichia coli and use in polymerase chain reaction amplification. Protein Expression and Purification, 16, 96-102.
• Hassl A., Müller W.A., Aspöck H. 1991. An identical epitope in Pneumocystis carinii and Toxoplasma gondii causing serological cross-reactions. Parasitology Research, 77, 351-352.
• Hiszczyńska-Sawicka E., Brillowska-Dąbrowska A., Dąbrowski S., Pietkiewicz H., Myjak P., Kur J. 2003. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expression and Purification, 27, 150-157.
• Huang X., Xuan X., Suzuki H., Sugimoto C., Nagasawa H., Fujisaki K., Mikami T., Igarashi I. 2002. Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clinical and Diagnostic Laboratory Immunology, 9, 1343-1347.
• Jacobs D., Vercammen M., Saman E. 1999. Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clinical and Diagnostic Laboratory Immunology, 6, 21-29.
• Lecordier L., Fourmaux M., Marcier C., Dehecq E., Masy E., Cesbron-Delauw M. 2000. Enzyme-linked immunosorbent assays using the recombinant dense granule antigens GRA6 and GRA1 of Toxoplasma gondii for detection of immunoglobulin G antibodies. Clinical and Diagnostic Laboratory Immunology, 7, 607-611.
• Li S., Galvan G., Araujo F.G., Suzuki Y., Remington J.S., Parmley S. 2000a. Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens. Clinical and Diagnostic Laboratory Immunology, 1, 781-787.
• Li S., Maine G., Suzuki Y., Araujo F.G., Galvan G., Remington J.S., Parmley S. 2000b. Serodiagnosis of recently acquired Toxoplasma gondii infection with a recombinant antigen. Journal of Clinical Microbiology, 38, 179-184.
• Martin V., Arcavi M., Santillan G., Amendoeira M.R.R., De Souza Neves E., Griemberg G., Guarnera E., Garberi J.C., Angel S.O. 1998. Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii Rop2 protein. Clinical and Diagnostic Laboratory Immunology, 5, 627-631.
• Parmley S.F., Sgarlato G.D., Mark J., Prince J.B., Remington J.S. 1992. Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii. Journal of Clinical Microbiology, 30, 1127-1133.
• Pietkiewicz H., Hiszczyńska-Sawicka E., Kur J., Petersen E., Nielsen H.V., Stankiewicz M., Andrzejewska I., Myjak P. 2004. Usefulness of Toxoplasma gondii-specific recombinant antigens in serodiagnosis of human toxoplasmosis. Journal of Clinical Microbiology, 42, 1779-1781.
• Redlich A., Müller W. A. 1998. Serodiagnosis of acute toxoplasmosis using a recombinant form of the dense granule antigen GRA6 in an enzyme-linked immunosorbent assay. Parasitology Research, 84, 700-706.
• Tenter A.M., Johnson A.M. 1991. Recognition of recombinant Toxoplasma gondii antigens by human sera in an ELISA. Parasitology Research, 77, 197-203.