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2006 | 62 | 01 |

Tytuł artykułu

Zmiany morfometryczne ukladu rozrodczego loszek podczas mikotoksykozy zearalenonowej

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Oestrus status without standing reflex is enumerated among the typical clinical signs of zearalenone mycotoxicosis in sexually immature gilts. The aim of the study was to assess the influence of a low dose of zearalenone applied per os for 7 days on the morphometric results of the organs of the reproductive system in sexually immature gilts that were and that were not subjected to ovariectomy. Zearalenone and α-zearalenole were determined in blood plasma with the common use of separation methods with the columns of immunological affinity and high performance liquid chromatography with fluorescent detection. Tissues of the reproductive system were taken directly after the slaughter (on day 8 of the experiment) for morphometric examinations. The study was carried out on twelve 4 months old gilts, at 38-45 kg of body weight. The results obtained showed that per os application of zearalenone at a dose of 200 µg /kg b.w. for 7 days evoked signs of apparent sexual readiness without standing reflex in gilts after ovariectomy and in physiologically efficient gilts. The level of α-zearalenole had been significantly increased and zearalenone had been noted in blood serum before these signs appeared. Double and triple enlargement of the uterus was noted in both experimental groups. The mass and volume of the ovaries in physiologically efficient gilts diminished about 50%.

Wydawca

-

Rocznik

Tom

62

Numer

01

Opis fizyczny

s.99-102,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Warminsko-Mazurski, ul. Oczapowskiego 2, 10-718 Olsztyn
autor
autor
autor
autor

Bibliografia

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  • 2.Bao B., Kumar N., Karp R. M., Garverick H. A., Sundaram K.: Estrogen Receptor-β Expression in Relation to the Expression of Luteinizing Hormone Receptor and Cytochrome P450 Enzymes in Rat Ovarian Follicles. Biol. Reprod. 2000, 63, 1747-1755.
  • 3.Belstra B. A., Diekman M. A., Richert B. T., Singleton W. L.: Effects of lactation length and an exogenous progesterone and estradiol-17β regimen during embryo attachment on endogenous steroid concentrations and embryo survival in sows. Theriogenology 2002, 57, 2063-2081.
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  • 6.Eertmans F., Dhooge W., Stuyvaert S., Comhaire F.: Endocrine disruptors: effects on male fertility and screening tools for their assessment. Toxicol. in Vitro 2003, 17, 515-524.
  • 7.Gajęcki M.: Zearalenone - undesirable substances in feed. Pol. J. Vet. Sci. 2002, 5, 117-122.
  • 8.Haritha S., Rajagopalan G.: Follicular growth, endometrial thickness, and serum estradiol levels in spontaneous and clomiphene citrate-induced cycles. Int. J. Gynecol. Obstet. 2003, 81, 287-292.
  • 9.Harlow C. R., Hillier S. G.: Connective tissue growth factor in the ovarian paracrine system. Mol. Cell. Endocrinol. 2002, 187, 23-27.
  • 10.Jóźwiak J., Podgórski J.: Statystyka od podstaw. Polskie Wydawnictwo Ekonomiczne. Warszawa 2000.
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  • 12.Kotwica J., Krzymowski T., Dębek J.: Kaniulizowanie naczyń żylnych u świń do badań endokrynologicznych. Medycyna Wet. 1978, 44, 118-120.
  • 13.Krzysiek J., Gregoraszczuk E., Milewicz T., Klimek R.: Kontrola wzrostu i atrezji pęcherzyków jajnikowych. Gin. Prakt. 1996, 4, 39-41.
  • 14.Madej J. A.: Estrogeny w nowotworach narządów płciowych. Medycyna Wet. 2004, 60, 6-11.
  • 15.Marsden D. E., Hacker N. F.: The classification, diagnosis and management of endometrial hyperplasia. Rev. Gynecol. Pract. 2003, 3, 89-97.
  • 16.Mayr U., Butsch A., Schneider S.: Validation of two in vitro test systems for estrogenic activities with zearalenone, phytoestrogens and cereal extracts. Toxicology 1992, 74, 135-149.
  • 17.Mikamo E., Harada S., Nishikawa J., Nishihara T.: Endocrine disruptors induce cytochrome P450 by affecting transcriptional regulation via pregnane X receptor. Toxicol. Appl. Pharmacol. 2003, 193, 66-72.
  • 18.Mueller S. O.: Overview of in vitro tools to assess the estrogenic and antiestrogenic activity of phytoestrogens. J. Chromatogr. B 2002, 777, 155-165.
  • 19.Mueller S., Simon S., Chae K., Metzler M., Korach K. S.: Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor α (ERα) and ERβ in human cells. Toxicol. Sci. 2004, 80, 14-25.
  • 20.Olsen M. E., Pettersson H., Sandholm K. A., Kiessling K. C.: Quantitative liquid chromatographic method using fluorescence detection for determining zearalenone and its metabolites in blood plasma and urine. J. Assoc. Anal. 1985, 68, 632-635.
  • 21.Ouanes Z., Ayed-Boussema I., Baati T., Creppy E. E., Bacha H.: Zearalenone induces chromosome aberrations in mouse bone marrow: preventive effect of 17-estradiol, progesterone and Vitamin E. Mutat. Res-Gen. Tox. En. 2005, 565, 139-149.
  • 22.Ptaszyńska M.: Hormonalna regulacja cyklu owulacyjnego, ciąży i porodu u świń. Medycyna Wet. 2001, 76, 641-645.
  • 23.Rosenfeld C. S., Wagner J. S., Roberts R. M., Lubahn D. B.: Intraovarian actions of estrogen. Reproduction 2001, 122, 215-226.
  • 24.Roulillier P., Matton P., Dufour M., Sirard M. A., Guilbault L. A.: Steroid production, cell proliferation, and apoptosis in cultured bovine antral and mural granulose cells: development of an in vitro model to study estradiol production. Mol. Reprod. Dev. 1998, 50, 170-177.
  • 25.Słomczyńska M.: Dynamika rozmieszczenia receptorów hormonów steroidowych w jajniku. Postępy Biol. Kom. 2002, 29, 27-46.
  • 26.Słomczyńska M.: The effect of phytoestrogens on the reproductive tract. Pol. J. Vet. Sci. 2004, 7, 223-226.
  • 27.Słomczyńska M., Woźniak J.: Differential distribution of estrogen receptor -β and estrogen receptor -α in the porcine ovary. Exp. Clin. Endocrinol. Diabetes 2001, 109, 238-244.
  • 28.Tiemann U., Tomek W., Schneider F., Vanselow J.: Effects of mycotoxins α-and β-zearalenol on regulation of progesterone synthesis in cultured granulose cells from porcine ovaries. Reprod. Toxicol. 2003, 17, 673-681.
  • 29.Tiemann U., Viergutz T., Jonas L., Schneider F.: Influence of the mycotoxins α- and β-zearalenol and deoxynivalenol on the cell cycle of cultured porcine endometrial cells. Reprod. Toxicol. 2003, 17, 209-218.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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