Indukowana aksotomia plastycznosc neuronow czuciowych zaopatrujacych zwoj krezkowy doogonowy swini

• Autorzy: Bossowska A , Skobowiat C. , Gonkowski S. , Wojtkiewicz J. , Majewski M.

Warianty tytułu

EN

Axotomy-induced plasticity of sensory neurons supplying porcine caudal mesenteric ganglions

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to establish whether the dorsal root ganglion neurons supplying the porcine CaMG contain SP and/or CGRP and, additionally, which changes in the expression pattern of these peptides may be induced by a mechanical injury applied to the processes of the above neurons. The study was carried out on consecutive frozen serial sections of DRG taken from 12 eight-week old pigs, in which the neuronal tracer Fast Blue (FB) had been injected three weeks previously into the right CaMG. Six animals were then randomly chosen for ipsilateral ganglionectomy. Eventual changes in the chemical phenotypes of the injured cells were studied a week later using routine double-immunofluorescence labeling. FB+ neurons contained SP and CGRP (32% and 42%, respectively). The vast majority of SP- and CGRP-IR afferent cells belonged to the class of medium-sized (64% and 59%, respectively) and small neurons (32% and 37%, respectively). A co-localization of SP and CGRP was observed in 22% of FB+ neurons. The resection of CaMG resulted in a dramatic increase in the number of FB+ cells containing SP (55%) and a statistically significant decrease in the number of CGRP-IR neurons associated with CaMG (29%). These results suggest that sensory neurons associated with porcine CaMG contain SP and CGRP and that a re-section of CaMG is able to induce profound changes in the expression pattern of the studied peptides, implying deep mechanical injury-induced adaptative changes in the studied afferent neurons.

Słowa kluczowe

PL

peptyd CGRP; substancja P; plastycznosc; trzoda chlewna; neurony czuciowe; zwoj krezkowy doogonowy

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 10
• Opis fizyczny: s.1167-1170,fot.,bibliogr.

Twórcy

autor

Bossowska A

• Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 13, 10-719 Olsztyn

autor

Skobowiat C.

autor

Gonkowski S.

autor

Wojtkiewicz J.

autor

Majewski M.

Bibliografia

• 1.Alm P., Lundberg L. M.: Co-existence and origin of peptidergic and adrenergic nerves in the guinea pig uterus. Retrograde tracing and immunocytochemistry, effects of chemical sympathectomy, capsaicin treatment and pregnancy. Cell Tissue Res. 1988, 254, 517-530.
• 2.Bonfanti L., Bellardi S., Ghidella S., Gobetto A., Polak J. M., Merighi A.: Distribution of five peptides, three general neuroendocrine markers, and two synaptic-vesicle-associated proteins in the spinal cord and dorsal root ganglia of the adult and newborn dog: an immunocytochemical study. Am. J. Anat. 1991, 191, 154-166.
• 3.Bossowska A.: Distribution of primary afferent neurons associated with the porcine inferior mesenteric ganglion (IMG). Folia Histochem. Cytobiol. 2002, 40, 367-372.
• 4.Dalsgaard C. J., Elfvin L. G.: Structural studies on the connectivity of the inferior mesenteric ganglion of the guinea pig. J. Auton. Nerv. Syst. 1982, 5, 265-278.
• 5.Dalsgaard C. J., Hökfelt T., Elfvin L. G., Skirboll L., Emson P.: Substance P-containing primary sensory neurons projecting to the inferior mesenteric ganglion: evidence from combined retrograde tracing and immunohistochemistry. Neuroscience 1982, 7, 647-654.
• 6.Dalsgaard C. J., Hökfelt T., Schultzberg M., Lundberg J. M., Terenius L., Dockray G. J., Goldstein M.: Origin of peptide-containing fibers in the inferior mesenteric ganglion of the guinea-pig: immunohistochemical studies with antisera to substance P, enkephalin, vasoactive intestinal polypeptide, cholecystokinin and bombesin. Neuroscience 1983, 9, 191-211.
• 7.Dun N. J., Jiang Z. G.: Non-cholinergic excitatory transmission in inferior mesenteric ganglia of the guinea-pig: possible mediation by substance P. J. Physiol. 1982, 325, 145-159.
• 8.Elfvin L. G., Dalsgaard C. J.: Retrograde axonal transport of horseradish perioxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. Identification of the cells of origin in dorsal root ganglia. Brain Res. 1977, 126, 149-153.
• 9.Garry M. G., Miller K. E., Seybold V. S.: Lumbar dorsal root ganglia of the cat: a quantitative study of peptide immunoreactivity and cell size. J. Comp. Neurol. 1989, 284, 36-47.
• 10.Gibbins I. L., Furness J. B., Costa M.: Pathway-specific patterns of the co-existence of substance P, calcitonin gene-related peptide, cholecystokinin and dynorphin in neurons of the dorsal root ganglia of the guinea-pig. Cell Tissue Res. 1987, 248, 417-437.
• 11.Hökfelt T., Elde R., Johansson O., Luft R., Nilsson G., Arimura A.: Immunohistochemical evidence for separate populations of somatostatin-containing and substance P-containing primary afferent neurons in the rat. Neuroscience 1976, 1, 131-136.
• 12.Hökfelt T., Kellerth J. O., Nilsson G., Pernow B.: Substance P: Localization in the central nervous system and in some primary sensory neurons. Science 1975, 190, 889-890.
• 13.Hökfelt T., Zhang X., Wiesenfeld-Hallin Z.: Messenger plasticity in primary sensory neurons following axotomy and its functional implications. Trends Neurosci. 1994, 17, 22-30.
• 14.Inaishi Y., Kashihara Y., Sakaguchi M., Nawa H., Kuno M.: Cooperative regulation of calcitonin gene-related peptide levels in rat sensory neurons via their central and peripheral processes. J. Neurosci. 1992, 12, 518-524.
• 15.Kaleczyc J., Scheuermann D. W., Pidsudko Z., Majewski M., Lakomy M., Timmermans J.-P.: Distribution, immunohistochemical characteristic and nerve pathways of primary sensory neurons supplying the porcine vas deferens. Cell Tissue Res. 2002, 310, 9-17.
• 16.Krier J., Schmalz P. F., Szurszewski J. H.: Central innervation of neurones in the inferior mesenteric ganglion and of the large intestine of the cat. J. Physiol. 1982, 332, 125-138.
• 17.Kruger L., Silverman J. D., Mantyh P., Sternini C., Brecha N. C.: Peripheral patterns of calcitonine gene-related peptide general somatic sensory innervation: cutaneous and deep terminations. J. Comp. Neurol. 1989, 280, 291-302.
• 18.Le Greves P., Nyberg F., Hökfelt T., Terenius L.: Calcitonin gene-related peptide is metabolized by an endopeptidase hydrolyzing substance P. Regul. Pept. 1989, 25, 277-286.
• 19.Majewski M.: Synaptogenesis and structure of the autonomic ganglia. Folia Morphol. 2000, 58, 65-99.
• 20.Matthews M. R., Cuello A. C.: Substance P-immunoreactive peripheral branches of sensory neurons innervate guinea pig sympathetic neurons. Proc. Natl Acad. Sci. USA 1982, 79, 1668-1672.
• 21.McCarthy P. W., Lawson S. N.: Cell type and conduction velocity of rat primary sensory neurons with substance P-like immunoreactivity. Neuroscience 1989, 28, 745-753.
• 22.Oku R., Satoh M., Fujii N., Otaka A., Yajima H., Takagi H.: Calcitonin gene-related peptide promotes mechanical nociception by potentiating release of substance P from the spinal dorsal horn in rats. Brain Res. 1987, 403, 350-354.
• 23.Papka R. E., Thompson B. D., Schmidt H. H.: Identification of uterine-related sympathetic neurons in the rat inferior mesenteric ganglion: neurotransmitter content and afferent input. J. Auton. Nerv. Syst. 1996, 59, 51-59.
• 24.Ryu P. D., Murase K., Gerber G., Randic M.: Actions of calcitonin generelated peptide on rat sensory ganglion neurones. Physiol. Bohemoslov. 1988, 37, 259-265.
• 25.Supowit S. C., Ethridge R. T., Zhao H., Katki K. A., DiPette D. J.: Calcitonin gene-related peptide and substance P contribute to reduced blood pressure in sympathectomized rats. Am. J. Physiol. 2005, 289, H1169-H1175.
• 26.Tessler A., Himes B. T., Krieger N. R., Murray M., Goldberger M. E.: Sciatic nerve transection produces death of dorsal root ganglion cells and reversible loss of substance P in spinal cord. Brain Res. 1985, 332, 209-218.
• 27.Wong J., Oblinger M. M.: NGF rescues substance P expression but not neurofilament or tubulin gene expression in axotomized sensory neurons. J. Neurosci. 1991, 11, 543-552.
• 28.Zheng Z. L., Travagli R. A., Kreulen D. L.: Patterns of innervation of sympathetic vascular neurons by peptide-containing primary sensory fibers. Brain Res. 1999, 827, 113-121.