Transient and stable transformation of wheat with DNA preparations delivered by a biolistic method

• Autorzy: Dobrzanska M , Krysiak C. , Kraszewska E.
• Języki publikacji: EN

Abstrakty

EN

An optimized procedure for transformation of wheat with the use of a Biolistic Particle Delivery System PDS 1000/He to deliver foreign DNA is described in detail. The bacterial uidA and bar genes (both driven by plant promoters) were utilized as the reporter and selectable marker genes, respectively. Moderately high gas pressure appeared to be most important to achieve the highest level of transient GUS expression in target tissues. There was, however, no apparent correlation between transient and stable GUS expression. The presence of telomeric DNA sequences in an uidA gene-containing vector did not influence transient GUS expression but, apparently, prevented its stable expression. Mechanical lesions caused by the bombardment (tungsten particles) seemed to be less severe when embryo- derived calli, instead of freshly excised immature embryos, were used as the target tissue. The limited ability of callus cells for regeneration, together with a restricted number of cells that receive the foreign DNA by particle bombardment, result in a low efficiency of wheat stable transformation.

Słowa kluczowe

EN

transformation; Biolistic Particle Delivery System; biolistic method; gene expression; wheat; Triticum aestivum; embryogenesis; DNA

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 3
• Opis fizyczny: p.277-284,fig.

Twórcy

autor

Dobrzanska M

• Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Krysiak C.

autor

Kraszewska E.

Bibliografia

• Becker D., Brettschneider R., Lortz H. 1994. Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J. 5: 299–307.
• Blechl A.E., Anderson O.D. 1996. Expression of a novel high-molecular-weight glutenin subunit gene in transgenic wheat. Nature Biotechnology 14: 875–879.
• Buchole M., Buchowicz J. 1994. A minichromosome-like structure in wheat embryos. Plant Mol. Biol. Rep. 12: 204–208.
• Chiurazzi M., Signer E.R. 1994. Termini and telomeres in T-DNA transformation. Plant Mol. Biol. 26: 923–934.
• Jefferson R.A. 1987. Assaying chimeric genes in plants: the GUS gene system. Plant Mol. Biol. Rep. 5: 387–405.
• Klein T.M., Wolf E.D., Wu R., Sanford J.C. 1987. High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73.
• Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
• Nehra N.S., Chibbar R.N., Leung N., Caswell K., Mallard C., Steinhauer L., Baga M., Kartha B. 1994. Self fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J. 5: 285–297.
• Perl A., Kless H., Blumenthal A., Galli G., Galun E. 1992. Improvement of plant regeneration and GUS expression in scutellar wheat calli by optymization of culture conditions and DNA-microprojectile delivery procedures. Mol Gen. Genet. 235: 279–284.
• Sambrook J., Fritsch E.R., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
• Sanford J.C., Smith F.D., Russel J.A. 1993. Optimizing the biolistic process for different biological applications. Methods Enzymol. 217: 483–509.
• Szurmak B., Buchowicz J. 1997. Autonomous replication of wheat DNA sequence in isolated wheat nuclei. Acta Biochim. Polon. 44: 79–82.
• Vasil I.K. 1994. Molecular improvement of cereals. Plant Mol. Biol. 25: 925–937.
• Vasil V., Castillo A.M., Fromm M.E., Vasil I.K. 1992. Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Biotechnology 10: 667–674.
• Weeks J.T., Anderson O.D., Blechl A.E. 1993. Rapid production of multiple idependent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102: 1077–1084.
• Zhang W., McElroy D., Wu R. 1991. Analysis of rice Act1 5′ region activity in transgenic rice plants. Plant Cell 3: 1155–1165.
• Zimny J., Becker D., Brettschneider R., Lorz H. 1995. Fertile, transgenic Triticale (x Triticosecale Wittmack). Mol. Breeding 1: 155–164.