Characteristics of adhesive properties of Lactobacillus strains synthesising biosurfactants

• Autorzy: Golek P , Bednarski W. , Lewandowska M.

Warianty tytułu

PL

Charakterystyka wlasciwosci adhezyjnych szczepow Lactobacillus syntetyzujacych biosurfaktanty

• Języki publikacji: EN

Abstrakty

EN

This study provides an assessment of the adhesive properties of Lactobacillus spp. strains which synthesise biosurfactants to a mono monolayer of intestinal epithelium in vitro, represented by the Caco-2 cell line. It also examines the effect of impregnating a polystyrene surface with a solution of biosurfactants synthesised by the Lactobacillus casei rhamnosus CCM 1825 strain on the adhesion of Klebsiella pneumoniae 2. All tested Lactobacillus strains showed adhesion to Caco-2 cells. The highest adhesion was observed for the Lactobacillus fermenti 126 strain: 3000 cells to 1000 Caco-2 cells or 420.000 cells to 1 cm² of the intestinal epithelium, while the lowest was for the Lactobacillus casei rhamnosus CCM 1825, whose 2400 and 310.000 cells adhered to 1000 cells or 1 cm² of Caco-2 tissue, were determined respectively. A 50% reduction in the population of Klebsiella pneumoniae 2 cells adhering to the surface previously impregnated with a solution of biosurfactants synthesised by Lactobacillus casei rhamnosus CCM 1825, after the 3-hour contact with the tested surface was also observed.

PL

Oceniano właściwości przylegania szczepów z rodzaju Lactobacillus syntetyzujących biosurfaktanty do monowarstwy komórek nabłonka jelitowego in vitro reprezentowanej przez linię komórkową Caco-2. Testowano także wpływ impregnacji powierzchni polisterynowej roztworem biosurfaktantów syntetyzowanych przez szczep Lactobacillus casei rhamnosus CCM 1825 na przyleganie bakterii Klebsiella pneumoniae 2. Wszystkie testowane szczepy Lactobacillus przylegały do komórek Caco-2. Największą adhezję wykazano w przypadku szczepu Lactobacillus fermenti 126 (3000 komórek L. fermenti przylegało do 1000 komórek Caco-2 lub 420 000 komórek do 1 cm² tkanki nabłonka jelitowego), a najmniejszą w przypadku Lactobacillus casei rhamnosus CCM 1825 (2400 oraz 310 000 komórek L. casei rhamnosus przylegało odpowiednio do 1000 komórek lub 1 cm² tkanki Caco-2). Ponadto wykazano 50% redukcję populacji komórek Klebsiella pneumoniae 2 przylegających do powierzchni wcześniej impregnowanej roztworem biosurfaktantów syntetyzowanych przez Lactobacillus casei rhamnosus CCM 1825, po ich 3 h kontakcie z testowaną powierzchnią.

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2007
• Tom: 22
• Numer: 2
• Opis fizyczny: p.333-342,fig.,ref.

Twórcy

autor

Golek P

• University of Warmia and Mazury in Olsztyn, Jana Heweliusza 1, 10-724 Olsztyn, Poland

autor

Bednarski W.

autor

Lewandowska M.

Bibliografia

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