Porownanie metodami AP-PCR, rybotypowania [PCR-ribotyping] oraz elektroforezy pulsacyjnej [PFGE] szczepow Clostridium difficile wytwarzajacych toksyne B i nie wytwarzajacych toksyny A

• Autorzy: Pituch H. , Van Belkum A. , Obuch-Woszczatynski P. , Meisel-Mikolajczyk F. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Do porównania szczepów Clostridium difficile z delecją w genie kodującym toksynę A zastosowano metodę AP-PCR, rybotypowania (PCR-ribotyping) oraz elektroforezy pulsacyjnej (PFGE). Zbadano dziewięć szczepów, które nie wytwarzają toksyny A a wytwarzają toksynę В [tox A(-) tox B(+)]. Szczepy wyhodowano z próbek kału pobranych od pacjentów z biegunką poantybiotykową, leczonych w różnych oddziałach szpitalnych i w różnym czasie. Z przeprowadzonych badań wynika, że szczepy C. difficile z delecją w genie toksyny A, izolowane w Polsce, stanowią homogenną pod względem genetycznym grupę szczepów.

EN

In this study were used AP-PCR, PCR-ribotyping and pulsed -field ele-crophoresis (PFGE) for compartive study of toxin A-negative /toxin B-posi-tive Clostridium difficile strains with deletion in toxin A gen. We investigated nine unrelated clinical strains, isolated from different units and different time from patients suffering to antibiotic associated diarrhea (AAD). We found that toxin A-negative/ toxin B-positive C. difficile strains isolated in Poland belonging to a single genotyp A, are being similar to the Japanese strains.

Słowa kluczowe

PL

elektroforeza pulsacyjna; toksyna A; mikrobiologia lekarska; Clostridium difficile; biegunki; lancuchowa reakcja polimerazy; zakazenia szpitalne; mikroorganizmy chorobotworcze; patogennosc; toksyna B

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 1
• Opis fizyczny: s.53-60,rys.,tab.,bibliogr.

Twórcy

autor

Pituch H.

• Akademia Medyczna, Warszawa

autor

Van Belkum A.

autor

Obuch-Woszczatynski P.

autor

Meisel-Mikolajczyk F.

autor

Luczak M.

Bibliografia

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