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2009 | 60 | 2 |
Tytuł artykułu

Modulation of inflammatory response by pentoxifylline is independent of heme oxygenase-1 pathway

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Abstrakty
EN
Objective: It was reported that some effects of pentoxifylline (PTX) are mediated by heme oxygenase-1 (HO-1) induction. We investigated the role of HO-1 in anti-inflammatory activity of PTX. Methods: Experiments were performed in human and murine monocytes and endothelial cells and in HO-1 deficient mice. Results: PTX dose-dependently decreased expression of HO-1 in cell lines studied. As expected, PTX reduced also production of TNF. This effect was independent of HO-1 activity, as demonstrated in cells treated with HO-1 activators and inhibitors or in cells overexpressing HO-1. Moreover, inhibition of TNF was the same in human endothelial cells of different HO-1 genotypes, showing that PTX is similarly efficient in carriers of more and less active HO-1 promoter variants. In mice, PTX did not influence HO-1 expression, as measured in liver, kidney, spleen, heart, and skin. Accordingly, the response of PTX treated animals to LPS was the same in wild type and HO-1 deficient mice. PTX to a similar extent increased influx of leukocyte into peritoneal cavity, decreased production of TNF and reduced expression of VCAM-1 in vascular intima. Conclusion: PTX inhibits production of TNF and may decrease inflammatory reaction both in vitro and in vivo, but these effects are independent of HO-1.
Wydawca
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Rocznik
Tom
60
Numer
2
Opis fizyczny
p.3-12,fig.,ref.
Twórcy
autor
  • Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
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Bibliografia
Typ dokumentu
Bibliografia
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bwmeta1.element.agro-article-9f12ae1b-e006-47ae-9df4-3254e3444c14
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