Wykrywanie wirusa choroby pecherzykowej swin w materiale biologicznym

• Autorzy: Paprocka G

Warianty tytułu

EN

Detection of swine vesicular disease virus [SVDV] in biological material

• Języki publikacji: PL

Abstrakty

EN

Swine vesicular disease virus (SVDV) is a member of the genus Enterovirus in the family Picornaviridae. This virus appears to have evolved from human coxsackievirus B5. Pigs infected with this virus show almost identical clinical signs to foot-and-mouth disease in pigs. Vesicular diseases must be differentiated with laboratory tests. The purpose of the study was to apply the isolation test in cell culture and RT-PCR assay for the detection of swine vesicular disease virus in the epithelial and fecal samples. Out of a total of 11 examined samples, 10 were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of isolation assay. Primary piglet kidney cells and certain IB-RS-2 cells were a sensitive cell culture system for the detection of swine vesicular disease virus, whereas secondary lamb kidney cells not.

Słowa kluczowe

PL

trzoda chlewna; choroby zwierzat; choroba pecherzykowa swin; wirus choroby pecherzykowej swin; wykrywanie; izolacja; test izolacji wirusa; metoda RT-PCR

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 02
• Opis fizyczny: s.118-120,fot.,tab.,bibliogr.

Twórcy

autor

Paprocka G

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy w Pulawach, Zaklad Pryszczycy, ul.Wodna 7, 98-220 Zdunska Wola

Bibliografia

• 1.Anon.: OIE Manual of Diagnostic Test and Vaccines for Terrestrial Animals (mammals, birds and bees), Sixth Edition 2008, 1139-1145.
• 2.Escribano-Romero E., Jimenez-Clavero M. A., Ley V.: Swine vesicular disease virus. Pathology of the disease and molecular characteristics of the virion. Anim. Health. Res. Rev. 2000, 1, 119-126.
• 3.Jimenez-Clavero M. A., Escribano-Romero E., Ley V., Spiller O. B.: More recent swine vesicular disease virus isolates reatain binding to coxsackie-adenovirus receptor, but have lost the ability to bind human decay-accelerating factor (CD55). J. Gen. Virol. 2005, 86, 1369-1377.
• 4.Knowles N. J., Wadsworth J., Reid S. M., Swabey K. G., El-kholy A. A., Abd El-Rahman A. O., Soliman H. M., Ebert K., Ferris N. P., Hutchings G. H., Statham R. J., King D. P., Paton D. J.: Reappearance of swine vesicular disease virus in Portugal. Emerg. Infect. Dis. 2007, 13, 1593-1596.
• 5.Lin F., Mackay D. K. J., Knowles N. J.: Detection of swine vesicular disease virus RNA by reverse transcription-polymerase chain reaction. J. Virol. Methods 1997, 65, 111-121.
• 6.Nunez J. I., Blanco E., Hernandez T., Gomex-Tejedor C., Martin M. I., Dopazo J., Sobrino F.: A RT-PCR assay for the differential diagnosis of vesicular viral diseases of swine. J. Virol. Methods 1998, 72, 227-235.
• 7.Paprocka G.: Izolacja i identyfikacja wirusa pryszczycy. Medycyna Wet. 2008, 64, 1404-1406.
• 8.Paprocka G.: Zastosowanie hodowli pierwotnej komórek nerki jagnięcia w diagnostyce pryszczycy. Medycyna Wet. 2008, 64, 332-334.
• 9.Reid S. M., Ferris N. P., Hutchings G. H., Zhang Z., Belsham G. J., Alexandersen S.: Sensitivity of primary cells immortalised by oncogene transfection for the detection and isolation of foot-and-mouth disease and swine vesicular disease viruses. J. Virol. Methods 2002, 105, 67-80.
• 10.Verdaguer N., Jimenez-Clavero M. A., Fita I., Ley V.: Structure of swine vesicular disease virus: mapping of changes occurring during adaptation of human coxsackie B5 virus to infect swine. J. Virol. 2003, 77, 9780-9789.