Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report

• Autorzy: Styczynski J. , Wysocki M. , Debski R. , Balwierz W. , Rokicka-Milewska R. , Matysiak M. , Balcerska A. , Kowalczyk J. , Wachowiak J. , Sonta-Jakimczyk D. , Chybicka A.
• Języki publikacji: EN

Abstrakty

EN

In vitro antileukemic activity of five glucocorticoids and their cross-resistance pat­tern in childhood acute lymphoblastic and non-lymphoblastic leukemia were deter­mined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 uM hydrocortisone (HC), 8 uM prednisolone (PRE), 1.5 uM methylprednisolone (MPR), 0.44 uM dexamethasone (DX) and 0.22 Mbetamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX (> 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). TheAMLcell samples were even more resistant to: PRE (>85-fold, p=0.001), DX (> 34-fold, p = 0.004),MPR(> 69-fold, p = 0.036), BET (> 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A significant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.

Słowa kluczowe

EN

glucocorticoid resistance; leukemia; acute leukemia; sensitivity; childhood; in vitro; cross resistance

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.93-98

Twórcy

autor

Styczynski J.

• Medical University, Bydgoszcz, Poland

autor

Wysocki M.

autor

Debski R.

autor

Balwierz W.

autor

Rokicka-Milewska R.

autor

Matysiak M.

autor

Balcerska A.

autor

Kowalczyk J.

autor

Wachowiak J.

autor

Sonta-Jakimczyk D.

autor

Chybicka A.

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