Modulation of CCK-8-evoked intracellular Ca2+ waves by hydrogen peroxide in mouse pancreatic acinar cells

• Autorzy: Granados M.P. , Salido G.M. , Pariente J.A. , Gonzalez A.
• Języki publikacji: EN

Abstrakty

EN

In the present study we have employed single cell imaging analysis to monitor the propagation of cholecystokinin-evoked Ca2+ waves in mouse pancreatic acinar cells. Stimulation of cells with 1 nM CCK-8 led to an initial Ca2+ release at the luminal cell pole and subsequent spreading of the Ca2+ signal towards the basolateral membrane in the form of a Ca2+ wave. Inhibition of sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) activity by 1 µM thapsigargin, preincubation in the presence of 100 µM H2O2 or inhibition of PKC with either 5 µM Ro31-8220 or 3 µM GF-109203-X all led to a faster propagation of CCK-8-induced Ca2+ signals. The propagation of CCK-8-evoked Ca2+ signals was slowed down by activation of PKC with 1 µM PMA, and preincubation of cells in the presence of H2O2 counteracted the effect of PKC inhibition. The protonophore FCCP (100 nM) and the inhibitor of the mitochondrial Ca2+-uniporter Ru360 (10 µM) led to an increase in the propagation rate of CCK-8-evoked Ca2+ waves. Finally, depolymerisation of actin cytoskeleton with cytochalasin D (10 µM) led to a faster propagation of CCK-8-evoked Ca2+ signals. Stabilization of actin cytoskeleton with jasplakinolide (10 µM) did not induce significant changes on CCK-8-evoked Ca2+ waves. Preincubation of cells in the presence of H2O2 counteracted the effect of cytochalasin D on CCK-8-evoked Ca2+ wave propagation. Our results suggest that spreading of cytosolic Ca2+ waves evoked by CCK-8 can be modulated by low levels of oxidants acting on multiple Ca2+-handling mechanisms.

Słowa kluczowe

EN

mouse; mice; fluorescence; hydrogen peroxide; pancreatic acinar cell; calcium wave; imaging analysis; cholecystokinin

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 3
• Opis fizyczny: p.423-440,fig.,ref.

Twórcy

autor

Granados M.P.

• University of Extremadura, Avenida Universidad s/n, E-10071, Caceres, Spain

autor

Salido G.M.

autor

Pariente J.A.

autor

Gonzalez A.