Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

• Autorzy: Micieta K , Stano J. , Korenova M. , Blanarikova V. , Fulmekova M. , Matejka P.
• Języki publikacji: EN

Abstrakty

EN

A simple and rapid procedure for identification and determination of extracellular saccha- rase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspen­sion cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.

PL

W pracy opisano prostą i szybką metodę identyfikacji i oznaczania sacharazy zewnątrz- komórkowej z użyciem pożywki z kultur zawiesin komórkowych glistnika jaskółcze ziele (Chelidonium majus L.). Substrátem użytym do określenia zewnątrzkomórkowej i wewnątrz­komórkowej aktywności badanego enzymu była sacharoza. Pożywkę (bez komórek) użyto do identyfikacji i oznaczenia zewnątrzkomórkowej aktywności enzymu. Aktywność we­wnątrzkomórkowa oznaczona była przy użyciu zawiesin komórkowych.

Słowa kluczowe

EN

cell suspension culture; determination; Chelidonium majus; extracellular saccharase; celandine; identification

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2005
• Tom: 51
• Numer: 1-2
• Opis fizyczny: p.54-59,fig.,ref.

Twórcy

autor

Micieta K

• Comenius University, Revova 39, 811 02 Bratislava, Slovak Republic

autor

Stano J.

autor

Korenova M.

autor

Blanarikova V.

autor

Fulmekova M.

autor

Matejka P.

Bibliografia

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